Human Serum Amyloid A1 /SAA1 ELISA Kit

$350.00$450.00

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TARGETSSAA1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.45 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery95% – 117%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.45 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery95% – 117%
Average Recovery1.04
Intra Precision3.5% – 4.8%
Inter-Precision4.7% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-SAA1 monoclonal antibody
Human SAA1 freeze-dried standard
HRP标记的抗Human SAA1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human SAA1单克隆 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, SAA1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of SAA1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

SAA1

Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Lung Disease (Asthma & COPD)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Sepsis
Systemic Lupus Erythematosus
Transplantation

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