Human sIL-2Rα/CD25 ELISA Kit

$350.00$450.00

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TARGETSIL2RA
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity2.99 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery87% – 104%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity2.99 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery87% – 104%
Average Recovery0.97
Intra Precision4.4% – 4.9%
Inter-Precision4.8% – 7.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sIL-2Rα/CD25 monoclonal antibody
Human sIL-2Rα/CD25 freeze-dried standard
sIL-2Rα/CD25 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sIL-2Rα antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sIL-2Rα present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sIL-2Rα in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL2RA

IL2RA Target Infomation Overview

  • Target Symbol: IL2RA, interleukin 2 receptor subunit alpha
  • Gene Groups: CD molecules; Interleukin receptors; Sushi domain containing
  • Alias: CD25
  • Previous Names: IL2R; IDDM10
  • Alias Names: insulin-dependent diabetes mellitus 10; interleukin 2 receptor, alpha

IL2RA, interleukin 2 receptor subunit alpha Target Infomation by Species

HumanMouseRat

Human IL2RA Target Information

  • Target Symbol: IL2RA, interleukin 2 receptor subunit alpha
  • Alias:
    • CD25
    • IDDM10
    • IL-2 receptor subunit alpha
    • IL-2-RA
    • IL-2R subunit alpha
    • IL2-RA
    • IL2R
    • IMD41
    • insulin-dependent diabetes mellitus 10
    • interleukin 2 receptor, alpha
    • interleukin 2 receptor, alpha chain
    • interleukin-2 receptor subunit alpha
    • p55
    • TAC antigen
    • TCGFR
  • NCBI_Gene: 3559
  • UniProtKB: P01589

Human IL2RA Predicted Functions

Contributes to interleukin-2 receptor activity. Involved in regulation of T cell tolerance induction. Predicted to be located in plasma membrane. Predicted to be part of interleukin-2 receptor complex. Implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Biomarker of COVID-19; Hantavirus hemorrhagic fever with renal syndrome; and idiopathic pulmonary fibrosis.

Mouse Il2ra Target Information

Mouse Il2ra Predicted Functions

Enables interleukin-2 binding activity and interleukin-2 receptor activity. Acts upstream of or within several processes, including Notch signaling pathway; activation-induced cell death of T cells; and regulation of T cell proliferation. Located in external side of plasma membrane. Is expressed in bone marrow. Used to study Sjogren’s syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Orthologous to human IL2RA (interleukin 2 receptor subunit alpha).

Rat Il2ra Target Information

  • Target Symbol: Il2ra, interleukin 2 receptor subunit alpha
  • Alias:
    • IL-2 receptor alpha subunit
    • IL-2 receptor subunit alpha
    • IL-2-RA
    • IL-2R subunit alpha
    • IL2-RA
    • IL2RAC
    • interleukin 2 receptor, alpha
    • interleukin 2 receptor, alpha chain
    • interleukin-2 receptor alpha chain
    • interleukin-2 receptor subunit alpha
  • NCBI_Gene: 25704
  • UniProtKB: P26897

Rat Il2ra Predicted Functions

Enables interleukin-2 receptor activity. Involved in inflammatory response to antigenic stimulus; positive regulation of T cell differentiation; and positive regulation of T cell proliferation. Located in cell surface. Used to study Korean hemorrhagic fever; asthma; and diabetes mellitus. Human ortholog(s) of this gene implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Orthologous to human IL2RA (interleukin 2 receptor subunit alpha).

IL2RA Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)

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