Mouse CXCL9/MIG ELISA Kit

$350.00$450.00

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TARGETSCXCL9
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.82 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery78% – 120%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.82 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery78% – 120%
Average Recovery0.98
Intra Precision2.1% – 4.5%
Inter-Precision3.8% – 6.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL9/MIG monoclonal antibody
Mouse CXCL9/MIG freeze-dried standard
CXCL9/MIG detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse CXCL9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CXCL9

CXCL9 Target Infomation Overview

  • Target Symbol: CXCL9, C-X-C motif chemokine ligand 9
  • Gene Groups: Chemokine ligands
  • Alias: SCYB9; Humig; crg-10
  • Previous Names: CMK; MIG
  • Alias Names: monokine induced by gamma interferon; chemokine (C-X-C motif) ligand 9

CXCL9, C-X-C motif chemokine ligand 9 Target Infomation by Species

HumanMouseRat

Human CXCL9 Target Information

  • Target Symbol: CXCL9, C-X-C motif chemokine ligand 9
  • Alias:
    • C-X-C motif chemokine 9
    • chemokine (C-X-C motif) ligand 9
    • CMK
    • crg-10
    • gamma-interferon-induced monokine
    • Humig
    • MIG
    • monokine induced by gamma interferon
    • monokine induced by interferon-gamma
    • SCYB9
    • small-inducible cytokine B9
  • NCBI_Gene: 4283
  • UniProtKB: Q07325

Human CXCL9 Predicted Functions

Enables CXCR3 chemokine receptor binding activity and chemokine activity. Involved in several processes, including adenylate cyclase-activating G protein-coupled receptor signaling pathway; antimicrobial humoral immune response mediated by antimicrobial peptide; and positive regulation of release of sequestered calcium ion into cytosol. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including chronic fatigue syndrome; cystic fibrosis; lung disease (multiple); severe acute respiratory syndrome; and thoracic aortic aneurysm.

Mouse Cxcl9 Target Information

Mouse Cxcl9 Predicted Functions

Predicted to enable CXCR3 chemokine receptor binding activity and chemokine activity. Acts upstream of or within several processes, including defense response to virus; positive regulation of myoblast differentiation; and positive regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in central nervous system; retina; stomach; testis; and thymus. Orthologous to human CXCL9 (C-X-C motif chemokine ligand 9).

Rat Cxcl9 Target Information

  • Target Symbol: Cxcl9, C-X-C motif chemokine ligand 9
  • Alias:
    • C-X-C motif chemokine 9
    • chemokine (C-X-C motif) ligand 9
    • MGC105312
    • Mig
    • monokine induced by gamma interferon
    • Scyb9
    • small inducible cytokine B9
  • NCBI_Gene: 246759
  • UniProtKB: Q8K4B1

Rat Cxcl9 Predicted Functions

Predicted to enable CXCR3 chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including cellular response to lipopolysaccharide; neutrophil chemotaxis; and positive regulation of release of sequestered calcium ion into cytosol. Predicted to act upstream of or within several processes, including defense response to virus; positive regulation of myoblast differentiation; and positive regulation of myoblast fusion. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Orthologous to human CXCL9 (C-X-C motif chemokine ligand 9).

CXCL9 Target News

    17.1
    Immunostimulant Hydrogel-Guided Tumor Microenvironment Reprogramming to Efficiently Potentiate Macrophage-Mediated Cellular Phagocytosis for Systemic Cancer Immunotherapy
    ACS Nano
    10
    Enhancement of Microglia Functions by Developed Nano-Immuno-Synergist to Ameliorate Immunodeficiency for Malignant Glioma Treatment
    Advanced Healthcare Materials
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis

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