Mouse CXCL9/MIG ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CXCL9
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.82 pg/mL
Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	78% – 120%
Average Recovery	0.98

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.82 pg/mL
Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	78% – 120%
Average Recovery	0.98
Intra Precision	2.1% – 4.5%
Inter-Precision	3.8% – 6.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL9/MIG monoclonal antibody Mouse CXCL9/MIG freeze-dried standard CXCL9/MIG detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse CXCL9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CXCL9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CXCL9

CXCL9 Target Infomation Overview

Target Symbol: CXCL9, C-X-C motif chemokine ligand 9
Gene Groups: Chemokine ligands
Alias: SCYB9; Humig; crg-10
Previous Names: CMK; MIG
Alias Names: monokine induced by gamma interferon; chemokine (C-X-C motif) ligand 9

CXCL9, C-X-C motif chemokine ligand 9 Target Infomation by Species

HumanMouseRat

Human CXCL9 Target Information

Target Symbol: CXCL9, C-X-C motif chemokine ligand 9
Alias:
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- CMK
- crg-10
- gamma-interferon-induced monokine
- Humig
- MIG
- monokine induced by gamma interferon
- monokine induced by interferon-gamma
- SCYB9
- small-inducible cytokine B9
NCBI_Gene: 4283
UniProtKB: Q07325

Human CXCL9 Predicted Functions

Enables CXCR3 chemokine receptor binding activity and chemokine activity. Involved in several processes, including adenylate cyclase-activating G protein-coupled receptor signaling pathway; antimicrobial humoral immune response mediated by antimicrobial peptide; and positive regulation of release of sequestered calcium ion into cytosol. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including chronic fatigue syndrome; cystic fibrosis; lung disease (multiple); severe acute respiratory syndrome; and thoracic aortic aneurysm.

Mouse Cxcl9 Target Information

Target Symbol: Cxcl9, chemokine (C-X-C motif) ligand 9
Alias:
- BB139920
- CMK
- crg-10
- expressed sequence BB139920
- Mig
- monokine induced by gamma interferon
- Scyb9
- small inducible cytokine B subfamily (Cys-X-Cys), member 9
NCBI_Gene: 17329

Mouse Cxcl9 Predicted Functions

Predicted to enable CXCR3 chemokine receptor binding activity and chemokine activity. Acts upstream of or within several processes, including defense response to virus; positive regulation of myoblast differentiation; and positive regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in central nervous system; retina; stomach; testis; and thymus. Orthologous to human CXCL9 (C-X-C motif chemokine ligand 9).

Rat Cxcl9 Target Information

Target Symbol: Cxcl9, C-X-C motif chemokine ligand 9
Alias:
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- MGC105312
- Mig
- monokine induced by gamma interferon
- Scyb9
- small inducible cytokine B9
NCBI_Gene: 246759
UniProtKB: Q8K4B1

Rat Cxcl9 Predicted Functions

Predicted to enable CXCR3 chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including cellular response to lipopolysaccharide; neutrophil chemotaxis; and positive regulation of release of sequestered calcium ion into cytosol. Predicted to act upstream of or within several processes, including defense response to virus; positive regulation of myoblast differentiation; and positive regulation of myoblast fusion. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Orthologous to human CXCL9 (C-X-C motif chemokine ligand 9).