Rat CCL3/MIP-1α ELISA Kit

$350.00$450.00

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TARGETSCCL3
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity0.09 pg/mL
Range6.25 pg/mL – 400 pg/mL
Assay Time3.5 h
Recovery90% – 119%
Average Recovery1.05
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity0.09 pg/mL
Range6.25 pg/mL – 400 pg/mL
Assay Time3.5 h
Recovery90% – 119%
Average Recovery1.05
Intra Precision4.8% – 5.0%
Inter-Precision3.5%-4.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL3/MIP-1α monoclonal antibody
Rat CCL3/MIP-1α freeze-dried standard
CCL3/MIP-1α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat CCL3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL3

CCL3 Target Infomation Overview

  • Target Symbol: CCL3, C-C motif chemokine ligand 3
  • Gene Groups: Chemokine ligands
  • Alias: G0S19-1; LD78ALPHA; MIP-1-alpha
  • Previous Names: SCYA3
  • Alias Names: small inducible cytokine A3 (homologous to mouse Mip-1a); chemokine (C-C motif) ligand 3

CCL3, C-C motif chemokine ligand 3 Target Infomation by Species

HumanMouseRat

Human CCL3 Target Information

  • Target Symbol: CCL3, C-C motif chemokine ligand 3
  • Alias:
    • C-C motif chemokine 3
    • chemokine (C-C motif) ligand 3
    • G0/G1 switch regulatory protein 19-1
    • G0S19-1
    • LD78 alpha beta
    • LD78ALPHA
    • macrophage inflammatory protein 1-alpha
    • MIP-1-alpha
    • MIP1A
    • PAT 464.1
    • SCYA3
    • SIS-beta
    • small inducible cytokine A3 (homologous to mouse Mip-1a)
    • small-inducible cytokine A3
    • tonsillar lymphocyte LD78 alpha protein
  • NCBI_Gene: 6348
  • UniProtKB: P10147

Human CCL3 Predicted Functions

Enables several functions, including calcium-dependent protein kinase C activity; chemoattractant activity; and chemokine receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and positive regulation of intracellular signal transduction. Located in cytosol and extracellular space. Implicated in human immunodeficiency virus infectious disease. Biomarker of COVID-19; alcoholic hepatitis; glomerulonephritis (multiple); kidney failure (multiple); and systemic scleroderma.

Mouse Ccl3 Target Information

Mouse Ccl3 Predicted Functions

Enables chemokine activity. Involved in several processes, including astrocyte cell migration; macrophage chemotaxis; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in articular cartilage; cartilage; knee joint; lung; and radius. Human ortholog(s) of this gene implicated in human immunodeficiency virus infectious disease. Orthologous to several human genes including CCL3 (C-C motif chemokine ligand 3).

Rat Ccl3 Target Information

  • Target Symbol: Ccl3, C-C motif chemokine ligand 3
  • Alias:
    • C-C motif chemokine 3
    • chemokine (C-C motif) ligand 3
    • macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)
    • macrophage inflammatory protein 1-alpha
    • MIP-1-alpha
    • MIP-1a
    • Scya3
    • small inducible cytokine A3
    • small-inducible cytokine A3
  • NCBI_Gene: 25542
  • UniProtKB: P50229

Rat Ccl3 Predicted Functions

Enables chemokine activity. Involved in several processes, including neutrophil chemotaxis; positive regulation of cytosolic calcium ion concentration; and positive regulation of osteoclast differentiation. Predicted to be located in cytosol and extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including Chagas disease; anterior uveitis; nephritis (multiple); renal fibrosis; and status epilepticus. Human ortholog(s) of this gene implicated in human immunodeficiency virus infectious disease. Orthologous to several human genes including CCL3 (C-C motif chemokine ligand 3).

CCL3 Target News

    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis

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