Rat HGF ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	HGF
SPECIES	Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	4.38 pg/mL
Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	81% – 126%
Average Recovery	0.92

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	4.38 pg/mL
Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	81% – 126%
Average Recovery	0.92
Intra Precision	3.0% – 3.7%
Inter-Precision	3.8% – 4.4%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- HGF monoclonal antibody Rat HGF freeze-dried standard HGF detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat HGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, HGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of HGF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

HGF

HGF Target Infomation Overview

Target Symbol: HGF, hepatocyte growth factor
Alias: SF; F-TCF; HGFB; HPTA
Previous Names: DFNB39
Alias Names: hepatopoietin A; fibroblast-derived tumor cytotoxic factor; scatter factor; lung fibroblast-derived mitogen; deafness, autosomal recessive 39; hepatocyte growth factor (hepapoietin A; scatter factor)

HGF, hepatocyte growth factor Target Infomation by Species

HumanMouseRat

Human HGF Target Information

Target Symbol: HGF, hepatocyte growth factor
Alias:
- deafness, autosomal recessive 39
- DFNB39
- F-TCF
- fibroblast-derived tumor cytotoxic factor
- hepatocyte growth factor (hepapoietin A, scatter factor)
- hepatopoeitin-A
- hepatopoietin-A
- HGFB
- HPTA
- lung fibroblast-derived mitogen
- SF
NCBI_Gene: 3082
UniProtKB: P14210

Human HGF Predicted Functions

Enables chemoattractant activity and identical protein binding activity. Involved in several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of programmed cell death; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling. Located in membrane. Implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Biomarker of several diseases, including artery disease (multiple); biliary tract disease (multiple); myositis (multiple); retinal disease (multiple); and rheumatoid arthritis (multiple).

Mouse Hgf Target Information

Target Symbol: Hgf, hepatocyte growth factor
Alias:
- C230052L06Rik
- HGF/SF
- NK1
- NK2
- RIKEN cDNA C230052L06 gene
- scatter factor
- SF/HGF
NCBI_Gene: 15234

Mouse Hgf Predicted Functions

Predicted to enable chemoattractant activity; growth factor activity; and identical protein binding activity. Acts upstream of or within several processes, including regulation of MAPK cascade; regulation of cytokine production; and regulation of protein phosphorylation. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; egg cylinder; embryo mesenchyme; and genitourinary system. Used to study autosomal recessive nonsyndromic deafness 39 and breast cancer. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Orthologous to human HGF (hepatocyte growth factor).

Rat Hgf Target Information

Target Symbol: Hgf, hepatocyte growth factor
Alias:
- hepatocyte growth factor (scatter factor)
- hepatopoeitin-A
- hepatopoietin-A
- HPTA
- scatter factor
- SF
NCBI_Gene: 24446
UniProtKB: P17945

Rat Hgf Predicted Functions

Enables growth factor activity. Involved in several processes, including animal organ regeneration; positive regulation of angiogenesis; and positive regulation of myelination. Located in extracellular space. Used to study brain infarction. Biomarker of cholangiocarcinoma. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Orthologous to human HGF (hepatocyte growth factor).