Rat HGF ELISA Kit

$350.00$450.00

TARGETS HGF
SPECIES Rat
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL(diluent)
Sensitivity 4.38 pg/mL
Range 125 pg/mL – 8000 pg/mL
Assay Time 3.5 h
Recovery 81% – 126%
Average Recovery 0.92
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL(diluent)
Sensitivity 4.38 pg/mL
Range 125 pg/mL – 8000 pg/mL
Assay Time 3.5 h
Recovery 81% – 126%
Average Recovery 0.92
Intra Precision 3.0% – 3.7%
Inter-Precision 3.8% – 4.4%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- HGF monoclonal antibody
Rat HGF freeze-dried standard
HGF detect Antibody
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat HGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, HGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of HGF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

HGF

HGF Target Infomation Overview

  • Target Symbol: HGF, hepatocyte growth factor
  • Alias: SF; F-TCF; HGFB; HPTA
  • Previous Names: DFNB39
  • Alias Names: hepatopoietin A; fibroblast-derived tumor cytotoxic factor; scatter factor; lung fibroblast-derived mitogen; deafness, autosomal recessive 39; hepatocyte growth factor (hepapoietin A; scatter factor)

HGF, hepatocyte growth factor Target Infomation by Species

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Human HGF Target Information

  • Target Symbol: HGF, hepatocyte growth factor
  • Alias:
    • deafness, autosomal recessive 39
    • DFNB39
    • F-TCF
    • fibroblast-derived tumor cytotoxic factor
    • hepatocyte growth factor (hepapoietin A, scatter factor)
    • hepatopoeitin-A
    • hepatopoietin-A
    • HGFB
    • HPTA
    • lung fibroblast-derived mitogen
    • SF
  • NCBI_Gene: 3082
  • UniProtKB: P14210

Human HGF Predicted Functions

Enables chemoattractant activity and identical protein binding activity. Involved in several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of programmed cell death; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling. Located in membrane. Implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Biomarker of several diseases, including artery disease (multiple); biliary tract disease (multiple); myositis (multiple); retinal disease (multiple); and rheumatoid arthritis (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Hgf Target Information

Mouse Hgf Predicted Functions

Predicted to enable chemoattractant activity; growth factor activity; and identical protein binding activity. Acts upstream of or within several processes, including regulation of MAPK cascade; regulation of cytokine production; and regulation of protein phosphorylation. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; egg cylinder; embryo mesenchyme; and genitourinary system. Used to study autosomal recessive nonsyndromic deafness 39 and breast cancer. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Orthologous to human HGF (hepatocyte growth factor).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Hgf Target Information

  • Target Symbol: Hgf, hepatocyte growth factor
  • Alias:
    • hepatocyte growth factor (scatter factor)
    • hepatopoeitin-A
    • hepatopoietin-A
    • HPTA
    • scatter factor
    • SF
  • NCBI_Gene: 24446
  • UniProtKB: P17945

Rat Hgf Predicted Functions

Enables growth factor activity. Involved in several processes, including animal organ regeneration; positive regulation of angiogenesis; and positive regulation of myelination. Located in extracellular space. Used to study brain infarction. Biomarker of cholangiocarcinoma. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Orthologous to human HGF (hepatocyte growth factor).

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HGF Target News

[catlist tags=”hgf” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Transplantation
Tumor Marker

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