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Human Apolipoprotein B/Apo B ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 74.26 pg/mL |
| Range | 0.31 ng/mL – 20 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 88% – 108% |
| Average Recovery | 0.98 |
| Intra Precision | 5.0% – 6.0% |
| Inter-Precision | 2.3%-6.1% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein B/Apo B monoclonal antibody Human Apolipoprotein B/Apo B freeze-dried standard Apolipoprotein B/Apo B detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo B antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo B present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo B in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
APOB
APOB Target Infomation Overview
- Target Symbol: APOB, apolipoprotein B
- Gene Groups: Apolipoproteins
- Alias Names: apolipoprotein B (including Ag(x) antigen)
APOB, apolipoprotein B Target Infomation by Species
HumanMouseRat
Human APOB Target Information
- Target Symbol: APOB, apolipoprotein B
- Alias:
- apo B-100
- apoB-100
- apoB-48
- apolipoprotein B (including Ag(x) antigen)
- apolipoprotein B-100
- apolipoprotein B48
- FCHL2
- FLDB
- LDLCQ4
- mutant Apo B 100
- NCBI_Gene: 338
- UniProtKB: P04114
Human APOB Predicted Functions
Enables several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Involved in several processes, including cholesterol homeostasis; low-density lipoprotein particle clearance; and low-density lipoprotein particle remodeling. Located in cytosol; endoplasmic reticulum exit site; and neuronal cell body. Part of low-density lipoprotein particle; mature chylomicron; and triglyceride-rich plasma lipoprotein particle. Implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Biomarker of several diseases, including common bile duct neoplasm; diabetes mellitus (multiple); familial combined hyperlipidemia; hypobetalipoproteinemia; and obesity.
Mouse Apob Target Information
- Target Symbol: Apob, apolipoprotein B
- Alias:
- AI315052
- apob-100
- apob-48
- expressed sequence AI315052
- NCBI_Gene: 238055
Mouse Apob Predicted Functions
Predicted to enable several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Acts upstream of or within several processes, including cholesterol efflux; lipoprotein metabolic process; and triglyceride metabolic process. Located in endoplasmic reticulum. Is expressed in several structures, including adrenal gland; brain; extraembryonic component; genitourinary system; and gut. Used to study familial hypobetalipoproteinemia 2. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Orthologous to human APOB (apolipoprotein B).
Rat Apob Target Information
- Target Symbol: Apob, apolipoprotein B
- Alias:
- Aa1064
- Ac1-060
- Apo B-100
- ApoB-100
- ApoB-48
- apolipoprotein B (including Ag(x) antigen)
- apolipoprotein B PI
- apolipoprotein B-100
- apolipoprotein B-48
- NCBI_Gene: 54225
Rat Apob Predicted Functions
Predicted to enable several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Involved in several processes, including cellular response to tumor necrosis factor; response to estradiol; and triglyceride catabolic process. Located in several cellular components, including extracellular space; vesicle lumen; and vesicle membrane. Part of high-density lipoprotein particle; low-density lipoprotein particle; and very-low-density lipoprotein particle. Biomarker of fatty liver disease and nephrotic syndrome. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Orthologous to human APOB (apolipoprotein B).
