Human Apolipoprotein B/Apo B ELISA Kit

$350.00$450.00

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TARGETSAPOB
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity74.26 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery88% – 108%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity74.26 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery88% – 108%
Average Recovery0.98
Intra Precision5.0% – 6.0%
Inter-Precision2.3%-6.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein B/Apo B monoclonal antibody
Human Apolipoprotein B/Apo B freeze-dried standard
Apolipoprotein B/Apo B detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo B antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo B present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo B in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

APOB

APOB Target Infomation Overview

  • Target Symbol: APOB, apolipoprotein B
  • Gene Groups: Apolipoproteins
  • Alias Names: apolipoprotein B (including Ag(x) antigen)

APOB, apolipoprotein B Target Infomation by Species

HumanMouseRat

Human APOB Target Information

  • Target Symbol: APOB, apolipoprotein B
  • Alias:
    • apo B-100
    • apoB-100
    • apoB-48
    • apolipoprotein B (including Ag(x) antigen)
    • apolipoprotein B-100
    • apolipoprotein B48
    • FCHL2
    • FLDB
    • LDLCQ4
    • mutant Apo B 100
  • NCBI_Gene: 338
  • UniProtKB: P04114

Human APOB Predicted Functions

Enables several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Involved in several processes, including cholesterol homeostasis; low-density lipoprotein particle clearance; and low-density lipoprotein particle remodeling. Located in cytosol; endoplasmic reticulum exit site; and neuronal cell body. Part of low-density lipoprotein particle; mature chylomicron; and triglyceride-rich plasma lipoprotein particle. Implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Biomarker of several diseases, including common bile duct neoplasm; diabetes mellitus (multiple); familial combined hyperlipidemia; hypobetalipoproteinemia; and obesity.

Mouse Apob Target Information

Mouse Apob Predicted Functions

Predicted to enable several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Acts upstream of or within several processes, including cholesterol efflux; lipoprotein metabolic process; and triglyceride metabolic process. Located in endoplasmic reticulum. Is expressed in several structures, including adrenal gland; brain; extraembryonic component; genitourinary system; and gut. Used to study familial hypobetalipoproteinemia 2. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Orthologous to human APOB (apolipoprotein B).

Rat Apob Target Information

Rat Apob Predicted Functions

Predicted to enable several functions, including cholesterol transfer activity; heparin binding activity; and lipase binding activity. Involved in several processes, including cellular response to tumor necrosis factor; response to estradiol; and triglyceride catabolic process. Located in several cellular components, including extracellular space; vesicle lumen; and vesicle membrane. Part of high-density lipoprotein particle; low-density lipoprotein particle; and very-low-density lipoprotein particle. Biomarker of fatty liver disease and nephrotic syndrome. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); biliary tract cancer (multiple); familial hyperlipidemia (multiple); hypobetalipoproteinemia (multiple); and sickle cell anemia. Orthologous to human APOB (apolipoprotein B).

APOB Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Obesity Diabetes

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