Human Apolipoprotein H/Apo H ELISA Kit

$350.00$450.00

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TARGETSAPOH
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity16.18 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery88% – 116%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity16.18 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery88% – 116%
Average Recovery1.03
Intra Precision2.4% – 6.3%
Inter-Precision3.9% – 4.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein H/Apo H monoclonal antibody
Human Apolipoprotein H/Apo H freeze-dried standard
Apolipoprotein H/Apo H detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo H antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate for incubation. After washing to remove unbound substances, the detection antibody labeled with horseradish peroxidase is added for further incubation. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo H in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

APOH

APOH Target Infomation Overview

  • Target Symbol: APOH, apolipoprotein H
  • Gene Groups: Apolipoproteins; Sushi domain containing
  • Alias: BG
  • Previous Names: B2G1
  • Alias Names: beta-2-glycoprotein I; apolipoprotein H (beta-2-glycoprotein I)

APOH, apolipoprotein H Target Infomation by Species

HumanMouseRat

Human APOH Target Information

  • Target Symbol: APOH, apolipoprotein H
  • Alias:
    • activated protein C-binding protein
    • anticardiolipin cofactor
    • APC inhibitor
    • apo-H
    • apolipoprotein H (beta-2-glycoprotein I)
    • B2G1
    • B2GP1
    • B2GPI
    • beta-2-glycoprotein 1
    • beta(2)GPI
    • BG
  • NCBI_Gene: 350
  • UniProtKB: P02749

Human APOH Predicted Functions

Enables identical protein binding activity; lipoprotein lipase activator activity; and phospholipid binding activity. Involved in several processes, including blood coagulation, intrinsic pathway; negative regulation of apoptotic process; and regulation of blood coagulation. Located in cell surface and extracellular space. Part of chylomicron; high-density lipoprotein particle; and very-low-density lipoprotein particle. Colocalizes with collagen-containing extracellular matrix. Implicated in antiphospholipid syndrome; myocardial infarction; and peripheral vascular disease. Biomarker of arteriosclerosis; diabetes mellitus; diabetic retinopathy; macular retinal edema; and type 2 diabetes mellitus.

Mouse Apoh Target Information

Mouse Apoh Predicted Functions

Predicted to enable identical protein binding activity; lipoprotein lipase activator activity; and phospholipid binding activity. Acts upstream of or within regulation of blood coagulation. Located in extracellular space. Is expressed in liver lobe and lung. Human ortholog(s) of this gene implicated in antiphospholipid syndrome; myocardial infarction; and peripheral vascular disease. Orthologous to human APOH (apolipoprotein H).

Rat Apoh Target Information

Rat Apoh Predicted Functions

Enables lipid binding activity. Involved in several processes, including negative regulation of respiratory burst; positive regulation of triglyceride catabolic process; and triglyceride transport. Located in several cellular components, including cell surface; extracellular space; and plasma membrane. Used to study myocardial infarction and thrombosis. Human ortholog(s) of this gene implicated in antiphospholipid syndrome; myocardial infarction; and peripheral vascular disease. Orthologous to human APOH (apolipoprotein H).

APOH Target News

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