Human Apolipoprotein M/Apo M ELISA Kit

$350.00$450.00

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TARGETSAPOM
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.44 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery90% – 109%
Average Recovery1
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.44 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery90% – 109%
Average Recovery1
Intra Precision2.1% – 3.9%
Inter-Precision3.4%-4.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein M/Apo M monoclonal antibody
Human Apolipoprotein M/Apo M freeze-dried standard
Human Apolipoprotein M/Apo M detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo M antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo M present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo M in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

APOM

APOM Target Infomation Overview

  • Target Symbol: APOM, apolipoprotein M
  • Gene Groups: Apolipoproteins; Lipocalins
  • Alias: G3a; NG20

APOM, apolipoprotein M Target Infomation by Species

HumanMouseRat

Human APOM Target Information

Human APOM Predicted Functions

Enables antioxidant activity; lipid transporter activity; and phospholipid binding activity. Involved in cholesterol efflux; high-density lipoprotein particle remodeling; and negative regulation of plasma lipoprotein oxidation. Part of high-density lipoprotein particle; low-density lipoprotein particle; and very-low-density lipoprotein particle. Implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus.

Mouse Apom Target Information

Mouse Apom Predicted Functions

Enables antioxidant activity. Involved in several processes, including cholesterol efflux; high-density lipoprotein particle clearance; and plasma lipoprotein particle organization. Acts upstream of or within lipoprotein metabolic process and reverse cholesterol transport. Located in extracellular region. Part of discoidal high-density lipoprotein particle. Is expressed in several structures, including gut; liver; male reproductive gland or organ; and urinary system. Human ortholog(s) of this gene implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus. Orthologous to human APOM (apolipoprotein M).

Rat Apom Target Information

Rat Apom Predicted Functions

Predicted to enable antioxidant activity; lipid transporter activity; and phospholipid binding activity. Involved in response to glucose. Located in extracellular space. Human ortholog(s) of this gene implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus. Orthologous to human APOM (apolipoprotein M).

APOM Target News

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