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Human Apolipoprotein M/Apo M ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 2.44 pg/mL |
| Range | 0.16 ng/mL – 10 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 90% – 109% |
| Average Recovery | 1 |
| Intra Precision | 2.1% – 3.9% |
| Inter-Precision | 3.4%-4.3% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein M/Apo M monoclonal antibody Human Apolipoprotein M/Apo M freeze-dried standard Human Apolipoprotein M/Apo M detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo M antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo M present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo M in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
APOM
APOM Target Infomation Overview
- Target Symbol: APOM, apolipoprotein M
- Gene Groups: Apolipoproteins; Lipocalins
- Alias: G3a; NG20
APOM, apolipoprotein M Target Infomation by Species
HumanMouseRat
Human APOM Target Information
- Target Symbol: APOM, apolipoprotein M
- Alias:
- alternative name: G3a, NG20
- apo-M
- G3a
- HSPC336
- MGC22400
- NG20
- NG20-like protein
- NCBI_Gene: 55937
- UniProtKB: O95445
Human APOM Predicted Functions
Enables antioxidant activity; lipid transporter activity; and phospholipid binding activity. Involved in cholesterol efflux; high-density lipoprotein particle remodeling; and negative regulation of plasma lipoprotein oxidation. Part of high-density lipoprotein particle; low-density lipoprotein particle; and very-low-density lipoprotein particle. Implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus.
Mouse Apom Target Information
- Target Symbol: Apom, apolipoprotein M
- Alias:
- 1190010O19Rik
- G3a
- NG20
- RIKEN cDNA 1190010O19 gene
- NCBI_Gene: 55938
Mouse Apom Predicted Functions
Enables antioxidant activity. Involved in several processes, including cholesterol efflux; high-density lipoprotein particle clearance; and plasma lipoprotein particle organization. Acts upstream of or within lipoprotein metabolic process and reverse cholesterol transport. Located in extracellular region. Part of discoidal high-density lipoprotein particle. Is expressed in several structures, including gut; liver; male reproductive gland or organ; and urinary system. Human ortholog(s) of this gene implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus. Orthologous to human APOM (apolipoprotein M).
Rat Apom Target Information
- Target Symbol: Apom, apolipoprotein M
- Alias:
- apo-M
- protein Px
- NCBI_Gene: 55939
- UniProtKB: P14630
Rat Apom Predicted Functions
Predicted to enable antioxidant activity; lipid transporter activity; and phospholipid binding activity. Involved in response to glucose. Located in extracellular space. Human ortholog(s) of this gene implicated in coronary artery disease; type 1 diabetes mellitus; and type 2 diabetes mellitus. Orthologous to human APOM (apolipoprotein M).
