Human C-Reactive Protein/CRP ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CRP
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.44 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	93% – 107%
Average Recovery	1

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.44 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	93% – 107%
Average Recovery	1
Intra Precision	2.9% – 5.5%
Inter-Precision	3.0% – 5.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti- CRP monoclonal antibody Human CRP freeze-dried standard CRP detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CRP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CRP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CRP in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CRP

CRP Target Infomation Overview

Target Symbol: CRP, C-reactive protein
Gene Groups: Short pentraxins
Alias: PTX1
Alias Names: pentraxin 1; C-reactive protein, pentraxin-related

CRP, C-reactive protein Target Infomation by Species

HumanMouseRat

Human CRP Target Information

Target Symbol: CRP, C-reactive protein
Alias:
- C-reactive protein, pentraxin-related
- c-reactive protein, petaxin related
- MGC149895
- MGC88244
- pentraxin 1
- PTX1
NCBI_Gene: 1401
UniProtKB: P02741

Human CRP Predicted Functions

Enables several functions, including complement component C1q complex binding activity; low-density lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including negative regulation of macrophage derived foam cell differentiation; positive regulation of superoxide anion generation; and regulation of gene expression. Acts upstream of or within vasoconstriction. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); autoimmune disease (multiple); eye disease (multiple); and lung disease (multiple).

Mouse Crp Target Information

Target Symbol: Crp, C-reactive protein, pentraxin-related
Alias:
- AI255847
- expressed sequence AI255847
NCBI_Gene: 12944

Mouse Crp Predicted Functions

Predicted to enable several functions, including cholesterol binding activity; complement component C1q complex binding activity; and low-density lipoprotein particle binding activity. Predicted to be involved in several processes, including complement activation, classical pathway; regulation of gene expression; and regulation of superoxide anion generation. Predicted to act upstream of or within vasoconstriction. Predicted to be located in filopodium and growth cone. Predicted to be active in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).

Rat Crp Target Information

Target Symbol: Crp, C-reactive protein
Alias:
- Aa1249
- Ab1-341
- Ab2-196
- Ac1-114
- Ac1262
- Ac2-069
- Ba2-693
- C-reactive protein member of the pentraxin family
- C-reactive protein, member of the pentraxin family
- C-reactive protein, pentraxin-related
- C-reactive protein, petaxin related
NCBI_Gene: 25419

Rat Crp Predicted Functions

Enables cholesterol binding activity; identical protein binding activity; and low-density lipoprotein particle binding activity. Involved in several processes, including cellular response to nitric oxide; complement activation, classical pathway; and negative regulation of superoxide anion generation. Located in extracellular space; filopodium; and growth cone. Used to study rheumatoid arthritis. Biomarker of several diseases, including acute kidney failure; artery disease (multiple); iron deficiency anemia; obesity; and status epilepticus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).