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Human CCL4/MIP-1β ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 3.58 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 122% |
| Average Recovery | 0.99 |
| Intra Precision | 1.8%-3.3% |
| Inter-Precision | 2.9% – 3.1% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL4/MIP-1β monoclonal antibody Human CCL4/MIP-1β freeze-dried standard CCL4/MIP-1β detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CCL4
CCL4 Target Infomation Overview
- Target Symbol: CCL4, C-C motif chemokine ligand 4
- Gene Groups: Chemokine ligands
- Alias: MIP-1-beta; Act-2; AT744.1
- Previous Names: LAG1; SCYA4
- Alias Names: small inducible cytokine A4 (homologous to mouse Mip-1b); chemokine (C-C motif) ligand 4
CCL4, C-C motif chemokine ligand 4 Target Infomation by Species
HumanMouseRat
Human CCL4 Target Information
- Target Symbol: CCL4, C-C motif chemokine ligand 4
- Alias:
- ACT2
- AT744.1
- C-C motif chemokine 4
- CC chemokine ligand 4
- chemokine (C-C motif) ligand 4
- G-26
- G-26 T-lymphocyte-secreted protein
- HC21
- LAG-1
- LAG1
- lymphocyte activation gene 1 protein
- lymphocyte-activation gene 1
- macrophage inflammatory protein 1-beta
- MGC104418
- MGC126025
- MGC126026
- MIP-1-beta
- MIP-1-beta(1-69)
- MIP1B
- MIP1B1
- PAT 744
- SCYA2
- SCYA4
- secreted protein G-26
- SIS-gamma
- small inducible cytokine A4 (homologous to mouse Mip-1b)
- small-inducible cytokine A4
- T-cell activation protein 2
- NCBI_Gene: 6351
- UniProtKB: P13236
Human CCL4 Predicted Functions
Enables CCR1 chemokine receptor binding activity; CCR5 chemokine receptor binding activity; and identical protein binding activity. Involved in several processes, including negative regulation by host of viral transcription; positive regulation of calcium-mediated signaling; and positive regulation of natural killer cell chemotaxis. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in acquired immunodeficiency syndrome. Biomarker of several diseases, including Plasmodium falciparum malaria; autoimmune disease (multiple); cystic fibrosis; lung disease (multiple); and nose disease (multiple).
Mouse Ccl4 Target Information
- Target Symbol: Ccl4, chemokine (C-C motif) ligand 4
- Alias:
- Act-2
- AT744.1
- macrophage inflammatory protein-1 beta
- MIP-1 beta
- MIP-1B
- Mip1b
- Scya4
- small inducible cytokine A4
- NCBI_Gene: 20303
Mouse Ccl4 Predicted Functions
Predicted to enable chemokine receptor binding activity and identical protein binding activity. Acts upstream of or within cellular response to xenobiotic stimulus. Located in extracellular space. Is expressed in central nervous system; genitourinary system; and retina. Human ortholog(s) of this gene implicated in acquired immunodeficiency syndrome and human immunodeficiency virus infectious disease. Orthologous to several human genes including CCL4L2 (C-C motif chemokine ligand 4 like 2).
Rat Ccl4 Target Information
- Target Symbol: Ccl4, C-C motif chemokine ligand 4
- Alias:
- C-C motif chemokine 4
- chemokine (C-C motif) ligand 4
- macrophage inflammatory protein 1-beta
- macrophage inflammatory protein-1 beta
- MIP-1-beta
- Mip1-b
- Scya4
- small inducible cytokine A4
- small-inducible cytokine A4
- NCBI_Gene: 116637
Rat Ccl4 Predicted Functions
Enables chemokine activity. Involved in several processes, including leukocyte chemotaxis; positive regulation of tumor necrosis factor production; and positive regulation of vascular permeability. Predicted to be active in extracellular space. Biomarker of anti-basement membrane glomerulonephritis; obesity; and status epilepticus. Human ortholog(s) of this gene implicated in acquired immunodeficiency syndrome and human immunodeficiency virus infectious disease. Orthologous to several human genes including CCL4L2 (C-C motif chemokine ligand 4 like 2).
