Product Category
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Human Chitinase 3-like 1/CHI3L1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL (prediluted) |
| Sensitivity | 1.10 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 110% |
| Average Recovery | 0.92 |
| Intra Precision | 1.9% – 2.6% |
| Inter-Precision | 3.2% – 5.0% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CHI3L1 monoclonal antibody Human CHI3L1 freeze-dried standard CHI3L1 detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CHI3L1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CHI3L1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of CHI3L1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CHI3L1
CHI3L1 Target Infomation Overview
- Target Symbol: CHI3L1, chitinase 3 like 1
- Gene Groups: Chitinases
- Alias: GP39; YKL40; YK-40
- Alias Names: cartilage glycoprotein-39; chitinase 3-like 1 (cartilage glycoprotein-39)
CHI3L1, chitinase 3 like 1 Target Infomation by Species
HumanMouseRat
Human CHI3L1 Target Information
- Target Symbol: CHI3L1, chitinase 3 like 1
- Alias:
- 39 kDa synovial protein
- ASRT7
- cartilage glycoprotein 39
- CGP-39
- chitinase 3-like 1 (cartilage glycoprotein-39)
- chitinase-3-like protein 1
- DKFZp686N19119
- FLJ38139
- GP-39
- GP39
- HC-gp39
- hCGP-39
- HCGP-3P
- YK-40
- YKL-40
- YKL40
- YYL-40
- NCBI_Gene: 1116
- UniProtKB: P36222
Human CHI3L1 Predicted Functions
Enables chitin binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; response to interleukin-1; and response to interleukin-6. Located in extracellular space. Implicated in asthma and schizophrenia. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bone cancer (multiple); lung disease (multiple); and reproductive organ cancer (multiple).
Mouse Chil1 Target Information
- Target Symbol: Chil1, chitinase-like 1
- Alias:
- AW208766
- Brp39
- Chi3l1
- chitinase 3-like 1
- expressed sequence AW208766
- glycoprotein 39
- Gp39
- NCBI_Gene: 12654
Mouse Chil1 Predicted Functions
Predicted to enable chitin binding activity. Involved in positive regulation of interleukin-8 production; positive regulation of protein kinase B signaling; and positive regulation of protein phosphorylation. Located in endoplasmic reticulum. Is expressed in alimentary system; limb; and skeleton. Human ortholog(s) of this gene implicated in asthma and schizophrenia. Orthologous to human CHI3L1 (chitinase 3 like 1).
Rat Chi3l1 Target Information
- Target Symbol: Chi3l1, chitinase 3 like 1
- Alias:
- cartilage glycoprotein 39
- CGP-39
- Chil1
- chitinase 3-like 1
- chitinase 3-like 1 (cartilage glycoprotein-39)
- chitinase-3-like protein 1
- GP-39
- MGC109420
- NCBI_Gene: 89824
Rat Chi3l1 Predicted Functions
Predicted to enable chitin binding activity. Involved in cellular response to interleukin-1; cellular response to tumor necrosis factor; and inflammatory response. Located in extracellular space. Biomarker of hypertension. Human ortholog(s) of this gene implicated in asthma and schizophrenia. Orthologous to human CHI3L1 (chitinase 3 like 1).
