Human Chitinase 3-like 1/CHI3L1 ELISA Kit

$350.00$450.00

Download:
TARGETSCHI3L1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL (prediluted)
Sensitivity1.10 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery80% – 110%
Average Recovery0.92
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL (prediluted)
Sensitivity1.10 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery80% – 110%
Average Recovery0.92
Intra Precision1.9% – 2.6%
Inter-Precision3.2% – 5.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CHI3L1 monoclonal antibody
Human CHI3L1 freeze-dried standard
CHI3L1 detect Antibody
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CHI3L1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CHI3L1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of CHI3L1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CHI3L1

CHI3L1 Target Infomation Overview

  • Target Symbol: CHI3L1, chitinase 3 like 1
  • Gene Groups: Chitinases
  • Alias: GP39; YKL40; YK-40
  • Alias Names: cartilage glycoprotein-39; chitinase 3-like 1 (cartilage glycoprotein-39)

CHI3L1, chitinase 3 like 1 Target Infomation by Species

HumanMouseRat

Human CHI3L1 Target Information

  • Target Symbol: CHI3L1, chitinase 3 like 1
  • Alias:
    • 39 kDa synovial protein
    • ASRT7
    • cartilage glycoprotein 39
    • CGP-39
    • chitinase 3-like 1 (cartilage glycoprotein-39)
    • chitinase-3-like protein 1
    • DKFZp686N19119
    • FLJ38139
    • GP-39
    • GP39
    • HC-gp39
    • hCGP-39
    • HCGP-3P
    • YK-40
    • YKL-40
    • YKL40
    • YYL-40
  • NCBI_Gene: 1116
  • UniProtKB: P36222

Human CHI3L1 Predicted Functions

Enables chitin binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; response to interleukin-1; and response to interleukin-6. Located in extracellular space. Implicated in asthma and schizophrenia. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); bone cancer (multiple); lung disease (multiple); and reproductive organ cancer (multiple).

Mouse Chil1 Target Information

Mouse Chil1 Predicted Functions

Predicted to enable chitin binding activity. Involved in positive regulation of interleukin-8 production; positive regulation of protein kinase B signaling; and positive regulation of protein phosphorylation. Located in endoplasmic reticulum. Is expressed in alimentary system; limb; and skeleton. Human ortholog(s) of this gene implicated in asthma and schizophrenia. Orthologous to human CHI3L1 (chitinase 3 like 1).

Rat Chi3l1 Target Information

Rat Chi3l1 Predicted Functions

Predicted to enable chitin binding activity. Involved in cellular response to interleukin-1; cellular response to tumor necrosis factor; and inflammatory response. Located in extracellular space. Biomarker of hypertension. Human ortholog(s) of this gene implicated in asthma and schizophrenia. Orthologous to human CHI3L1 (chitinase 3 like 1).

CHI3L1 Target News

    Multiple Sclerosis

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    Same Target