Human Follistatin ELISA Kit

$350.00$450.00

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TARGETSFST
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.99 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery87% – 125%
Average Recovery1.08
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.99 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery87% – 125%
Average Recovery1.08
Intra Precision4.0% – 5.2%
Inter-Precision4.5% – 6.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Follistatin monoclonal antibody
Human Follistatin freeze-dried standard
Follistatin detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Follistatin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Follistatin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Follistatin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

FST

FST Target Infomation Overview

  • Target Symbol: FST, follistatin
  • Alias: FS

FST, follistatin Target Infomation by Species

HumanMouseRat

Human FST Target Information

Human FST Predicted Functions

Enables activin binding activity and activin receptor antagonist activity. Involved in hematopoietic progenitor cell differentiation; negative regulation of activin receptor signaling pathway; and negative regulation of transcription by RNA polymerase II. Acts upstream of or within positive regulation of hair follicle development. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in polycystic ovary syndrome.

Mouse Fst Target Information

Mouse Fst Predicted Functions

Predicted to enable activin binding activity; activin receptor antagonist activity; and heparan sulfate proteoglycan binding activity. Acts upstream of or within several processes, including BMP signaling pathway; animal organ development; and keratinocyte proliferation. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; brain; early conceptus; embryo mesenchyme; and genitourinary system. Used to study lethal restrictive dermopathy. Human ortholog(s) of this gene implicated in polycystic ovary syndrome. Orthologous to human FST (follistatin).

Rat Fst Target Information

Rat Fst Predicted Functions

Enables heparan sulfate proteoglycan binding activity. Predicted to be involved in hematopoietic progenitor cell differentiation; negative regulation of transcription by RNA polymerase II; and regulation of transmembrane receptor protein serine/threonine kinase signaling pathway. Predicted to act upstream of or within several processes, including animal organ development; keratinocyte proliferation; and positive regulation of hair follicle development. Predicted to be located in cytoplasm and nucleus. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in polycystic ovary syndrome. Orthologous to human FST (follistatin).

FST Target News

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