Human M-CSF ELISA Kit

$350.00$450.00

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TARGETSCSF1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.19 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery81% – 116%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.19 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery81% – 116%
Average Recovery1.02
Intra Precision2.1% – 4.1%
Inter-Precision3.1% – 4.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-M-CSF monoclonal antibody
Human M-CSF freeze-dried standard
M-CSF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human M-CSF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the M-CSF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of M-CSF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CSF1

CSF1 Target Infomation Overview

  • Target Symbol: CSF1, colony stimulating factor 1
  • Alias: M-CSF; MCSF; MGC31930
  • Alias Names: macrophage colony stimulating factor 1; colony stimulating factor 1 (macrophage)

CSF1, colony stimulating factor 1 Target Infomation by Species

HumanMouseRat

Human CSF1 Target Information

  • Target Symbol: CSF1, colony stimulating factor 1
  • Alias:
    • colony stimulating factor 1 (macrophage)
    • CSF-1
    • lanimostim
    • macrophage colony stimulating factor
    • macrophage colony stimulating factor 1
    • macrophage colony-stimulating factor 1
    • MCSF
    • MGC31930
  • NCBI_Gene: 1435
  • UniProtKB: P09603

Human CSF1 Predicted Functions

Enables cytokine activity; growth factor activity; and protein homodimerization activity. Involved in several processes, including positive regulation of cell differentiation; positive regulation of macromolecule metabolic process; and positive regulation of macrophage migration. Acts upstream of or within positive regulation of cell population proliferation. Located in several cellular components, including extracellular space; nuclear body; and perinuclear region of cytoplasm. Implicated in interstitial cystitis. Biomarker of several diseases, including COVID-19; atherosclerosis; end stage renal disease; glomerulonephritis (multiple); and vesicoureteral reflux.

Mouse Csf1 Target Information

Mouse Csf1 Predicted Functions

Enables macrophage colony-stimulating factor receptor binding activity and protein homodimerization activity. Involved in several processes, including macrophage colony-stimulating factor signaling pathway; microglial cell proliferation; and positive regulation of gene expression. Acts upstream of or within several processes, including mammary gland development; osteoclast proliferation; and positive regulation of myeloid leukocyte differentiation. Located in extracellular space. Part of CSF1-CSF1R complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and hindlimb long bone. Used to study osteopetrosis. Human ortholog(s) of this gene implicated in interstitial cystitis. Orthologous to human CSF1 (colony stimulating factor 1).

Rat Csf1 Target Information

Rat Csf1 Predicted Functions

Enables identical protein binding activity. Involved in several processes, including myeloid leukocyte differentiation; odontogenesis; and ossification. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; high grade glioma; and osteopetrosis. Biomarker of systemic scleroderma. Human ortholog(s) of this gene implicated in interstitial cystitis. Orthologous to human CSF1 (colony stimulating factor 1).

CSF1 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis

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