Human MSP/MST1 ELISA Kit

$350.00$450.00

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TARGETSMST1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity1.33 pg/mL
Range39.06 pg/mL – 2500 pg/mL
Assay Time3.5 h
Recovery80% – 126%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity1.33 pg/mL
Range39.06 pg/mL – 2500 pg/mL
Assay Time3.5 h
Recovery80% – 126%
Average Recovery1.04
Intra Precision1.6% – 3.4%
Inter-Precision2.5%-3.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MSP monoclonal antibody
Human MSP freeze-dried standard
Human MSP detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MSP antibodies are precoated on a high-affinity ELISA plate.Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MSP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MSP in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MST1

MST1 Target Infomation Overview

  • Target Symbol: MST1, macrophage stimulating 1
  • Alias: MSP; NF15S2
  • Previous Names: D3F15S2; HGFL; DNF15S2
  • Alias Names: hepatocyte growth factor-like

MST1, macrophage stimulating 1 Target Infomation by Species

HumanMouseRat

Human MST1 Target Information

  • Target Symbol: MST1, macrophage stimulating 1
  • Alias:
    • D3F15S2
    • DNF15S2
    • hepatocyte growth factor-like protein
    • hepatocyte growth factor-like protein homolog
    • HGFL
    • macrophage stimulating 1 (hepatocyte growth factor-like)
    • macrophage stimulatory protein
    • macrophage-stimulating protein
    • MSP
    • NF15S2
  • NCBI_Gene: 4485
  • UniProtKB: P26927

Human MST1 Predicted Functions

Enables receptor tyrosine kinase binding activity. Involved in histone H2A-S139 phosphorylation; negative regulation of gluconeogenesis; and regulation of cAMP-dependent protein kinase activity. Located in extracellular space. Part of collagen-containing extracellular matrix.

Mouse Mst1 Target Information

Mouse Mst1 Predicted Functions

Predicted to enable receptor tyrosine kinase binding activity. Acts upstream of or within several processes, including embryo implantation; mammary duct terminal end bud growth; and positive regulation of mammary gland epithelial cell proliferation. Located in vacuole. Is expressed in esophagus epithelium; liver; liver parenchyma; pharynx epithelium; and stomach proventricular region epithelium. Orthologous to several human genes including MST1 (macrophage stimulating 1).

Rat Mst1 Target Information

Rat Mst1 Predicted Functions

Enables enzyme binding activity. Involved in cellular response to hypoxia; flagellated sperm motility; and spermatogenesis. Located in cytoplasm. Orthologous to several human genes including MST1 (macrophage stimulating 1).

MST1 Target News

    Tumor Marker

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