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Human myeloperoxidase/MPO ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 28.72 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 122% |
| Average Recovery | 1.04 |
| Intra Precision | 1.4% – 5.4% |
| Inter-Precision | 2.9% – 5.4% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Myeloperoxidase/MPO monoclonal antibody Human Myeloperoxidase/MPO freeze-dried standard Myeloperoxidase/MPO detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MPO antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MPO present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MPO in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
MPO
MPO Target Infomation Overview
- Target Symbol: MPO, myeloperoxidase
MPO, myeloperoxidase Target Infomation by Species
HumanMouseRat
Human MPO Target Information
- Target Symbol: MPO, myeloperoxidase
- Alias:
- 无
- NCBI_Gene: 4353
- UniProtKB: P05164
Human MPO Predicted Functions
Enables heparin binding activity and peroxidase activity. Involved in low-density lipoprotein particle remodeling. Acts upstream of or within hydrogen peroxide catabolic process. Located in azurophil granule; extracellular space; and nucleoplasm. Implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Biomarker of asthma (multiple); glomerulonephritis; and lung disease (multiple).
Mouse Mpo Target Information
- Target Symbol: Mpo, myeloperoxidase
- Alias:
- 无
- NCBI_Gene: 17523
Mouse Mpo Predicted Functions
Enables peroxidase activity. Acts upstream of or within several processes, including hypochlorous acid biosynthetic process; removal of superoxide radicals; and response to fungus. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; integumental system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).
Rat Mpo Target Information
- Target Symbol: Mpo, myeloperoxidase
- Alias:
- Mpo_mapped
- myeloperoxidase (mapped)
- NCBI_Gene: 303413
Rat Mpo Predicted Functions
Enables peroxidase activity. Involved in several processes, including response to food; response to gold nanoparticle; and response to lipopolysaccharide. Located in extracellular space. Used to study acute kidney failure. Biomarker of chronic obstructive pulmonary disease; cystitis; and renovascular hypertension. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).
