Human myeloperoxidase/MPO ELISA Kit

$350.00$450.00

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TARGETSMPO
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity28.72 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery80% – 122%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity28.72 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery80% – 122%
Average Recovery1.04
Intra Precision1.4% – 5.4%
Inter-Precision2.9% – 5.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Myeloperoxidase/MPO monoclonal antibody
Human Myeloperoxidase/MPO freeze-dried standard
Myeloperoxidase/MPO detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MPO antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MPO present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MPO in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MPO

MPO Target Infomation Overview

  • Target Symbol: MPO, myeloperoxidase

MPO, myeloperoxidase Target Infomation by Species

HumanMouseRat

Human MPO Target Information

Human MPO Predicted Functions

Enables heparin binding activity and peroxidase activity. Involved in low-density lipoprotein particle remodeling. Acts upstream of or within hydrogen peroxide catabolic process. Located in azurophil granule; extracellular space; and nucleoplasm. Implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Biomarker of asthma (multiple); glomerulonephritis; and lung disease (multiple).

Mouse Mpo Target Information

Mouse Mpo Predicted Functions

Enables peroxidase activity. Acts upstream of or within several processes, including hypochlorous acid biosynthetic process; removal of superoxide radicals; and response to fungus. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; integumental system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).

Rat Mpo Target Information

Rat Mpo Predicted Functions

Enables peroxidase activity. Involved in several processes, including response to food; response to gold nanoparticle; and response to lipopolysaccharide. Located in extracellular space. Used to study acute kidney failure. Biomarker of chronic obstructive pulmonary disease; cystitis; and renovascular hypertension. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).

MPO Target News

    2.7
    Stomatin promotes neutrophil degranulation and vascular leakage in the early stage of severe burn injury via enhancement of the intracellular binding of neutrophil primary granules to F-actin
    BURNS
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Transplantation

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