Mouse myeloperoxidase/MPO ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	MPO
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	4.40 pg/mL
Range	62.50 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	91% – 107%
Average Recovery	0.99

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	4.40 pg/mL
Range	62.50 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	91% – 107%
Average Recovery	0.99
Intra Precision	3.8% – 5.4%
Inter-Precision	3.0% – 4.1%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MPO monoclonal antibody Mouse MPO freeze-dried standard MPO detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse MPO antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MPO present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of MPO in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

MPO

MPO Target Infomation Overview

Target Symbol: MPO, myeloperoxidase

MPO, myeloperoxidase Target Infomation by Species

HumanMouseRat

Human MPO Target Information

Target Symbol: MPO, myeloperoxidase
Alias:
- 无
NCBI_Gene: 4353
UniProtKB: P05164

Human MPO Predicted Functions

Enables heparin binding activity and peroxidase activity. Involved in low-density lipoprotein particle remodeling. Acts upstream of or within hydrogen peroxide catabolic process. Located in azurophil granule; extracellular space; and nucleoplasm. Implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Biomarker of asthma (multiple); glomerulonephritis; and lung disease (multiple).

Mouse Mpo Target Information

Mouse Mpo Predicted Functions

Enables peroxidase activity. Acts upstream of or within several processes, including hypochlorous acid biosynthetic process; removal of superoxide radicals; and response to fungus. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; integumental system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).

Rat Mpo Target Information

Target Symbol: Mpo, myeloperoxidase
Alias:
- Mpo_mapped
- myeloperoxidase (mapped)
NCBI_Gene: 303413

Rat Mpo Predicted Functions

Enables peroxidase activity. Involved in several processes, including response to food; response to gold nanoparticle; and response to lipopolysaccharide. Located in extracellular space. Used to study acute kidney failure. Biomarker of chronic obstructive pulmonary disease; cystitis; and renovascular hypertension. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).