Mouse myeloperoxidase/MPO ELISA Kit

$350.00$450.00

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TARGETSMPO
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.40 pg/mL
Range62.50 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery91% – 107%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.40 pg/mL
Range62.50 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery91% – 107%
Average Recovery0.99
Intra Precision3.8% – 5.4%
Inter-Precision3.0% – 4.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MPO monoclonal antibody
Mouse MPO freeze-dried standard
MPO detect Antibody
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse MPO antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MPO present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of MPO in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MPO

MPO Target Infomation Overview

  • Target Symbol: MPO, myeloperoxidase

MPO, myeloperoxidase Target Infomation by Species

HumanMouseRat

Human MPO Target Information

Human MPO Predicted Functions

Enables heparin binding activity and peroxidase activity. Involved in low-density lipoprotein particle remodeling. Acts upstream of or within hydrogen peroxide catabolic process. Located in azurophil granule; extracellular space; and nucleoplasm. Implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Biomarker of asthma (multiple); glomerulonephritis; and lung disease (multiple).

Mouse Mpo Target Information

Mouse Mpo Predicted Functions

Enables peroxidase activity. Acts upstream of or within several processes, including hypochlorous acid biosynthetic process; removal of superoxide radicals; and response to fungus. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; integumental system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).

Rat Mpo Target Information

Rat Mpo Predicted Functions

Enables peroxidase activity. Involved in several processes, including response to food; response to gold nanoparticle; and response to lipopolysaccharide. Located in extracellular space. Used to study acute kidney failure. Biomarker of chronic obstructive pulmonary disease; cystitis; and renovascular hypertension. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human MPO (myeloperoxidase).

MPO Target News

    4.927
    Synthesis, Structural Elucidation, and Anti-Inflammatory Activity of a Water-Soluble Derivative of Arctiin
    MOLECULES
    4.927
    Study on the Comprehensive Phytochemicals and the Anti-Ulcerative Colitis Effect of Saussurea pulchella
    MOLECULES
    5.988
    Sirtuin 1 activator alleviated lethal inflammatory injury via promotion of autophagic degradation of pyruvate kinase M2
    Frontiers in Pharmacology
    4.9
    Schisandrin protects against ulcerative colitis by inhibiting the SGK1/NLRP3 signaling pathway and reshaping gut microbiota in mice
    Chinese Medicine
    7.3
    AhR-STAT3-Hmox1/Ptgs2 signaling pathway may restrict ferroptosis and improve hMSC accumulation and efficacy in mice liver
    BRITISH JOURNAL OF PHARMACOLOGY
    6.317
    Hesperetin attenuates sepsis-induced intestinal barrier injury by regulating neutrophil extracellular trap formation via the ROS/autophagy signaling pathway
    Food & Function
    5.5
    An X-ray inactivated vaccine against Pseudomonas aeruginosa Keratitis in mice
    VACCINE
    17.4
    Relieving thrombo-inflammation with acid-triggered polymersomes toward ischemic stroke therapy
    Nano Today
    3.1
    Light-induced circadian rhythm disorder leads to microvascular dysfunction via up-regulating NETs
    MICROVASCULAR RESEARCH
    5.195
    Huanglian-Houpo extract attenuates DSS-induced UC mice by protecting intestinal mucosal barrier and regulating macrophage polarization
    JOURNAL OF ETHNOPHARMACOLOGY
    5.195
    Shaoyao decoction restores the mucus layer in mice with DSS-induced colitis by regulating Notch signaling pathway
    JOURNAL OF ETHNOPHARMACOLOGY
    10.8
    Integration of MyD88 inhibitor into mesoporous cerium oxide nanozymes-based targeted delivery platform for enhancing treatment of ulcerative colitis
    JOURNAL OF CONTROLLED RELEASE
    5.6
    Effects of R-salbutamol on the inflammatory response and acute lung injury in endotoxemic mice
    INTERNATIONAL IMMUNOPHARMACOLOGY
    5.8
    Design, synthesis and characterization of a novel multicomponent salt of bexarotene with metformin and application in ameliorating psoriasis with T2DM
    INTERNATIONAL JOURNAL OF PHARMACEUTICS
    6.51
    Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy
    INTERNATIONAL JOURNAL OF PHARMACEUTICS
    8.025
    Folic acid-modified lactoferrin nanoparticles coated with a laminarin layer loaded curcumin with dual-targeting for ulcerative colitis treatment
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Transplantation

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