Human SP-D ELISA Kit

$350.00$450.00

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TARGETSSFTPD
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.45 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery94% – 120%
Average Recovery1.05
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.45 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery94% – 120%
Average Recovery1.05
Intra Precision5.2% – 6.1%
Inter-Precision3.2% – 8.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-SP-D monoclonal antibody
Human SP-D freeze-dried standard
SP-D detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human SP-D antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, SP-D present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of SP-D in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

SFTPD

SFTPD Target Infomation Overview

  • Target Symbol: SFTPD, surfactant protein D
  • Gene Groups: Collectins; C-type lectin domain containing
  • Alias: SP-D; COLEC7
  • Previous Names: SFTP4
  • Alias Names: surfactant, pulmonary-associated protein D

SFTPD, surfactant protein D Target Infomation by Species

HumanMouseRat

Human SFTPD Target Information

  • Target Symbol: SFTPD, surfactant protein D
  • Alias:
    • COLEC7
    • collectin 7
    • collectin-7
    • lung surfactant protein D
    • PSP-D
    • pulmonary surfactant apoprotein
    • pulmonary surfactant-associated protein D
    • SFTP4
    • SP-D
    • surfactant-associated protein, pulmonary 4
    • surfactant, pulmonary-associated protein D
  • NCBI_Gene: 6441
  • UniProtKB: P35247

Human SFTPD Predicted Functions

Predicted to enable identical protein binding activity. Involved in several processes, including induction of bacterial agglutination; negative regulation by host of symbiont catalytic activity; and surfactant homeostasis. Predicted to be located in cytoplasm. Predicted to be part of collagen trimer. Predicted to be active in extracellular space; multivesicular body; and rough endoplasmic reticulum. Implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Biomarker of several diseases, including cystic fibrosis; lung disease (multiple); respiratory syncytial virus infectious disease; sarcoidosis; and systemic lupus erythematosus.

Mouse Sftpd Target Information

  • Target Symbol: Sftpd, surfactant associated protein D
  • Alias:
    • AI573415
    • expressed sequence AI573415
    • Sfpd
    • Sftp4
    • SP-D
    • surfactant-associated protein 4
  • NCBI_Gene: 20390

Mouse Sftpd Predicted Functions

Enables identical protein binding activity. Predicted to be involved in several processes, including induction of bacterial agglutination; negative regulation of interleukin-2 production; and regulation of phagocytosis. Predicted to act upstream of or within innate immune response and respiratory gaseous exchange by respiratory system. Located in cytoplasm. Is expressed in lung; otic capsule; and skeleton. Human ortholog(s) of this gene implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Orthologous to human SFTPD (surfactant protein D).

Rat Sftpd Target Information

Rat Sftpd Predicted Functions

Enables identical protein binding activity; lipopolysaccharide binding activity; and monosaccharide binding activity. Involved in several processes, including negative regulation of interleukin-2 production; opsonization; and regulation of phagocytosis. Located in extracellular space; multivesicular body; and rough endoplasmic reticulum. Biomarker of Coronavirus infectious disease; asthma; bacterial pneumonia; lung disease; and newborn respiratory distress syndrome. Human ortholog(s) of this gene implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Orthologous to human SFTPD (surfactant protein D).

SFTPD Target News

    Lung Disease (Asthma & COPD)

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