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Human TIMP-3 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 1.74 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 97% – 116% |
| Average Recovery | 1.04 |
| Intra Precision | 4.3% – 4.9% |
| Inter-Precision | 4.7%-4.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-3 monoclonal antibody Human TIMP-3 freeze-dried standard Human TIMP-3 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TIMP3
TIMP3 Target Infomation Overview
- Target Symbol: TIMP3, TIMP metallopeptidase inhibitor 3
- Gene Groups: Tissue inhibitor of metallopeptidases
- Previous Names: SFD
- Alias Names: tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory)
TIMP3, TIMP metallopeptidase inhibitor 3 Target Infomation by Species
HumanMouseRat
Human TIMP3 Target Information
- Target Symbol: TIMP3, TIMP metallopeptidase inhibitor 3
- Alias:
- HSMRK222
- K222
- K222TA2
- metalloproteinase inhibitor 3
- MIG-5 protein
- protein MIG-5
- SFD
- TIMP metallopeptidase inhibitor 3 (Sorsby fundus dystrophy, pseudoinflammatory)
- TIMP-3
- tissue inhibitor of metalloproteinase 3
- tissue inhibitor of metalloproteinases 3
- NCBI_Gene: 7078
- UniProtKB: P35625
Human TIMP3 Predicted Functions
Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Involved in negative regulation of ERK1 and ERK2 cascade; negative regulation of membrane protein ectodomain proteolysis; and positive regulation of TRAIL-activated apoptotic signaling pathway. Located in nucleus. Colocalizes with collagen-containing extracellular matrix. Implicated in Sorsby’s fundus dystrophy; breast carcinoma; and urinary bladder cancer. Biomarker of several diseases, including breast cancer (multiple); preretinal fibrosis; reproductive organ cancer (multiple); thoracic aortic aneurysm; and type 2 diabetes mellitus.
Mouse Timp3 Target Information
- Target Symbol: Timp3, tissue inhibitor of metalloproteinase 3
- Alias:
- Timp-3
- NCBI_Gene: 21859
Mouse Timp3 Predicted Functions
Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Involved in negative regulation of metalloendopeptidase activity. Acts upstream of or within cellular response to organic substance. Located in basement membrane. Is expressed in several structures, including alimentary system; central nervous system; eye posterior segment; genitourinary system; and musculoskeletal system. Used to study Sorsby’s fundus dystrophy and dilated cardiomyopathy. Human ortholog(s) of this gene implicated in Sorsby’s fundus dystrophy; breast carcinoma; and urinary bladder cancer. Orthologous to human TIMP3 (TIMP metallopeptidase inhibitor 3).
Rat Timp3 Target Information
- Target Symbol: Timp3, TIMP metallopeptidase inhibitor 3
- Alias:
- metalloproteinase inhibitor 3
- TIMP-3
- tissue inhibitor of metalloproteinase 3
- tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory)
- tissue inhibitor of metalloproteinases 3
- NCBI_Gene: 25358
Rat Timp3 Predicted Functions
Predicted to enable metalloendopeptidase inhibitor activity; protease binding activity; and zinc ion binding activity. Involved in several processes, including negative regulation of proteolysis; response to estrogen; and response to folic acid. Located in cytoplasm. Human ortholog(s) of this gene implicated in Sorsby’s fundus dystrophy; breast carcinoma; and urinary bladder cancer. Orthologous to human TIMP3 (TIMP metallopeptidase inhibitor 3).
