Human TRAIL/TNFSF10 ELISA Kit

$350.00$450.00

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TARGETSTNFSF10
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity8.19 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery77% – 120%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity8.19 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery77% – 120%
Average Recovery0.97
Intra Precision6.6% – 8.3%
Inter-Precision4.8% – 6.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TRAIL/TNFSF10 monoclonal antibody
Human TRAIL/TNFSF10 freeze-dried standard
TRAIL/TNFSF10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TRAIL antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TRAIL present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TRAIL in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNFSF10

TNFSF10 Target Infomation Overview

  • Target Symbol: TNFSF10, TNF superfamily member 10
  • Gene Groups: CD molecules; Tumor necrosis factor superfamily
  • Alias: TRAIL; Apo-2L; TL2; CD253
  • Alias Names: tumor necrosis factor (ligand) superfamily, member 10

TNFSF10, TNF superfamily member 10 Target Infomation by Species

HumanMouseRat

Human TNFSF10 Target Information

  • Target Symbol: TNFSF10, TNF superfamily member 10
  • Alias:
    • Apo-2 ligand
    • Apo-2L
    • APO2L
    • CD253
    • chemokine tumor necrosis factor ligand superfamily member 10
    • TL2
    • TNF-related apoptosis inducing ligand TRAIL
    • TNF-related apoptosis-inducing ligand
    • TNLG6A
    • TRAIL
    • tumor necrosis factor (ligand) family, member 10
    • tumor necrosis factor (ligand) superfamily member 10
    • tumor necrosis factor (ligand) superfamily, member 10
    • tumor necrosis factor apoptosis-inducing ligand splice variant delta
    • tumor necrosis factor ligand 6A
    • tumor necrosis factor ligand superfamily member 10
    • tumor necrosis factor superfamily member 10
  • NCBI_Gene: 8743
  • UniProtKB: P50591

Human TNFSF10 Predicted Functions

Enables identical protein binding activity and zinc ion binding activity. Involved in positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of release of cytochrome c from mitochondria; and positive regulation of signal transduction. Located in extracellular exosome. Implicated in arteriosclerosis and small cell carcinoma. Biomarker of type 1 diabetes mellitus.

Mouse Tnfsf10 Target Information

Mouse Tnfsf10 Predicted Functions

Enables TRAIL binding activity and tumor necrosis factor receptor superfamily binding activity. Acts upstream of or within positive regulation of extrinsic apoptotic signaling pathway. Predicted to be located in extracellular space and plasma membrane. Predicted to be integral component of membrane. Is expressed in central nervous system; genitourinary system; gut; and retina. Human ortholog(s) of this gene implicated in arteriosclerosis and small cell carcinoma. Orthologous to human TNFSF10 (TNF superfamily member 10).

Rat Tnfsf10 Target Information

  • Target Symbol: Tnfsf10, TNF superfamily member 10
  • Alias:
    • 4-1BB
    • LOC500590
    • MGC114552
    • similar to T-cell antigen 4-1BB precursor – mouse
    • tumor necrosis factor receptor superfamily member 9
    • tumor necrosis factor receptor superfamily, member 9
  • NCBI_Gene: 246775
  • UniProtKB: E9PU84

Rat Tnfsf10 Predicted Functions

Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space and plasma membrane. Predicted to be active in external side of plasma membrane. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).

TNFSF10 Target News

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