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Human vWF ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 0.47 pg/mL |
| Range | 0.31 ng/mL – 20.00 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 96% – 107% |
| Average Recovery | 1.01 |
| Intra Precision | 2.4% – 6.1% |
| Inter-Precision | 3.7% – 5.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Human vWF monoclonal antibody Human vWF freeze-dried standard vWF detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human vWF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, vWF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of vWF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
VWF
VWF Target Infomation Overview
- Target Symbol: VWF, von Willebrand factor
- Gene Groups: Receptor ligands
- Previous Names: F8VWF
- Alias Names: Factor VIII related antigen
VWF, von Willebrand factor Target Infomation by Species
HumanMouseRat
Human VWF Target Information
- Target Symbol: VWF, von Willebrand factor
- Alias:
- coagulation factor VIII VWF
- F8VWF
- VWD
- NCBI_Gene: 7450
- UniProtKB: P04275
Human VWF Predicted Functions
Enables several functions, including chaperone binding activity; identical protein binding activity; and integrin binding activity. Involved in cell-substrate adhesion; platelet activation; and positive regulation of intracellular signal transduction. Located in several cellular components, including Weibel-Palade body; collagen-containing extracellular matrix; and endoplasmic reticulum. Implicated in several diseases, including Behcet’s disease; Bernard-Soulier syndrome; end stage renal disease; essential thrombocythemia; and von Willebrand’s disease (multiple). Biomarker of several diseases, including end stage renal disease; familial combined hyperlipidemia; obesity; osteonecrosis; and von Willebrand’s disease (multiple).
Mouse Vwf Target Information
- Target Symbol: Vwf, Von Willebrand factor
- Alias:
- 6820430P06Rik
- AI551257
- B130011O06Rik
- expressed sequence AI551257
- RIKEN cDNA 6820430P06 gene
- RIKEN cDNA B130011O06 gene
- NCBI_Gene: 22371
Mouse Vwf Predicted Functions
Predicted to enable several functions, including chaperone binding activity; identical protein binding activity; and integrin binding activity. Acts upstream of or within several processes, including liver development; placenta development; and platelet activation. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; extraembryonic component; genitourinary system; leptomeninges; and vitelline blood vessel. Used to study von Willebrand’s disease and von Willebrand’s disease 2. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Bernard-Soulier syndrome; end stage renal disease; essential thrombocythemia; and von Willebrand’s disease (multiple). Orthologous to human VWF (von Willebrand factor).
Rat Vwf Target Information
- Target Symbol: Vwf, von Willebrand factor
- Alias:
- von Willebrand factor homolog
- NCBI_Gene: 116669
Rat Vwf Predicted Functions
Predicted to enable several functions, including chaperone binding activity; identical protein binding activity; and integrin binding activity. Involved in several processes, including liver regeneration; platelet activation; and response to L-ascorbic acid. Located in collagen-containing extracellular matrix; endoplasmic reticulum; and extracellular space. Used to study transient cerebral ischemia. Biomarker of hypertension; mesangial proliferative glomerulonephritis; myocardial infarction; pulmonary fibrosis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Bernard-Soulier syndrome; end stage renal disease; essential thrombocythemia; and von Willebrand’s disease (multiple). Orthologous to human VWF (von Willebrand factor).
