Human vWF ELISA Kit

$350.00$450.00

Download:
TARGETSVWF
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.47 pg/mL
Range0.31 ng/mL – 20.00 ng/mL
Assay Time3.5 h
Recovery96% – 107%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.47 pg/mL
Range0.31 ng/mL – 20.00 ng/mL
Assay Time3.5 h
Recovery96% – 107%
Average Recovery1.01
Intra Precision2.4% – 6.1%
Inter-Precision3.7% – 5.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Human vWF monoclonal antibody
Human vWF freeze-dried standard
vWF detect Antibody
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human vWF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, vWF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of vWF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

VWF

VWF Target Infomation Overview

  • Target Symbol: VWF, von Willebrand factor
  • Gene Groups: Receptor ligands
  • Previous Names: F8VWF
  • Alias Names: Factor VIII related antigen

VWF, von Willebrand factor Target Infomation by Species

HumanMouseRat

Human VWF Target Information

Human VWF Predicted Functions

Enables several functions, including chaperone binding activity; identical protein binding activity; and integrin binding activity. Involved in cell-substrate adhesion; platelet activation; and positive regulation of intracellular signal transduction. Located in several cellular components, including Weibel-Palade body; collagen-containing extracellular matrix; and endoplasmic reticulum. Implicated in several diseases, including Behcet’s disease; Bernard-Soulier syndrome; end stage renal disease; essential thrombocythemia; and von Willebrand’s disease (multiple). Biomarker of several diseases, including end stage renal disease; familial combined hyperlipidemia; obesity; osteonecrosis; and von Willebrand’s disease (multiple).

Mouse Vwf Target Information

Mouse Vwf Predicted Functions

Predicted to enable several functions, including chaperone binding activity; identical protein binding activity; and integrin binding activity. Acts upstream of or within several processes, including liver development; placenta development; and platelet activation. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; extraembryonic component; genitourinary system; leptomeninges; and vitelline blood vessel. Used to study von Willebrand’s disease and von Willebrand’s disease 2. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Bernard-Soulier syndrome; end stage renal disease; essential thrombocythemia; and von Willebrand’s disease (multiple). Orthologous to human VWF (von Willebrand factor).

Rat Vwf Target Information

Rat Vwf Predicted Functions

Predicted to enable several functions, including chaperone binding activity; identical protein binding activity; and integrin binding activity. Involved in several processes, including liver regeneration; platelet activation; and response to L-ascorbic acid. Located in collagen-containing extracellular matrix; endoplasmic reticulum; and extracellular space. Used to study transient cerebral ischemia. Biomarker of hypertension; mesangial proliferative glomerulonephritis; myocardial infarction; pulmonary fibrosis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including Behcet’s disease; Bernard-Soulier syndrome; end stage renal disease; essential thrombocythemia; and von Willebrand’s disease (multiple). Orthologous to human VWF (von Willebrand factor).

VWF Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    EK1242

    $350.00$450.00

    EK1334

    $350.00$450.00

    EK2295

    $350.00$450.00

    EK1360

    $350.00$450.00

    EK2100

    $350.00$450.00

    $350.00$450.00

    EK1349

    $350.00$450.00

    $350.00$450.00

    $350.00$450.00

    EK2153

    $350.00$450.00

    $350.00$450.00

    EK2F01

    $350.00$450.00

    EK2313

    $350.00$450.00

    Same Target