Mouse IL-1α ELISA Kit

$350.00$450.00

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TARGETSIL1A
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.68 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery71% – 106%
Average Recovery0.89
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.68 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery71% – 106%
Average Recovery0.89
Intra Precision4.7% – 7.7%
Inter-Precision4.3% – 6.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1α monoclonal antibody
Mouse IL-1α freeze-dried standard
IL-1α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-1α antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-1α present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1α in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL1A

IL1A Target Infomation Overview

  • Target Symbol: IL1A, interleukin 1 alpha
  • Gene Groups: Interleukins
  • Alias: IL1F1; IL-1A; IL1-ALPHA
  • Previous Names: IL1
  • Alias Names: preinterleukin 1 alpha; hematopoietin-1; pro-interleukin-1-alpha

IL1A, interleukin 1 alpha Target Infomation by Species

HumanMouseRat

Human IL1A Target Information

  • Target Symbol: IL1A, interleukin 1 alpha
  • Alias:
    • hematopoietin-1
    • IL-1 alpha
    • IL-1A
    • IL1
    • IL1-ALPHA
    • IL1F1
    • interleukin 1, alpha
    • interleukin-1 alpha
    • preinterleukin 1 alpha
    • pro-interleukin-1-alpha
  • NCBI_Gene: 3552
  • UniProtKB: P01583

Human IL1A Predicted Functions

Enables copper ion binding activity and cytokine activity. Involved in several processes, including positive regulation of interleukin-2 production; positive regulation of mitotic nuclear division; and response to copper ion. Located in cytosol and extracellular space. Implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); cholesteatoma (multiple); common cold; cystic fibrosis; and osteoarthritis.

Mouse Il1a Target Information

Mouse Il1a Predicted Functions

Enables cytokine activity and interleukin-1 receptor binding activity. Involved in positive regulation of angiogenesis and positive regulation of vascular endothelial growth factor production. Acts upstream of or within several processes, including cell surface receptor signaling pathway; connective tissue replacement involved in inflammatory response wound healing; and positive regulation of cytokine production. Located in cytoplasm and extracellular space. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; reproductive system; and respiratory system. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Orthologous to human IL1A (interleukin 1 alpha).

Rat Il1a Target Information

Rat Il1a Predicted Functions

Predicted to enable copper ion binding activity; cytokine activity; and interleukin-1 receptor binding activity. Involved in several processes, including keratinization; negative regulation of establishment of Sertoli cell barrier; and positive regulation of intracellular signal transduction. Located in several cellular components, including cell surface; cytosol; and extracellular space. Used to study interstitial lung disease. Biomarker of several diseases, including endometritis; lung disease (multiple); urinary tract infection; uveitis; and varicocele. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Orthologous to human IL1A (interleukin 1 alpha).

IL1A Target News

    3.333
    Microglia PKM2 Mediates Neuroinflammation and Neuron Loss in Mice Epilepsy through the Astrocyte C3-Neuron C3R Signaling Pathway
    Brain Sciences
    6
    Oil control and pore refining effects of Fomes officinalis extract based on green deep eutectic solvent
    JOURNAL OF MOLECULAR LIQUIDS
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus

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