Human CCL2/MCP-1 ELISA Kit

$350.00$450.00

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TARGETSCCL2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.18 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery95% – 118%
Average Recovery1.07
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.18 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery95% – 118%
Average Recovery1.07
Intra Precision3.8% – 8.8%
Inter-Precision5.3% – 7.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL2/MCP-1 monoclonal antibody
Human CCL2/MCP-1 freeze-dried standard
CCL2/MCP-1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL2

CCL2 Target Infomation Overview

  • Target Symbol: CCL2, C-C motif chemokine ligand 2
  • Gene Groups: Chemokine ligands
  • Alias: MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
  • Previous Names: SCYA2
  • Alias Names: monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2, C-C motif chemokine ligand 2 Target Infomation by Species

HumanMouseRat

Human CCL2 Target Information

  • Target Symbol: CCL2, C-C motif chemokine ligand 2
  • Alias:
    • C-C motif chemokine 2
    • chemokine (C-C motif) ligand 2
    • GDCF-2
    • HC11
    • HSMCR30
    • MCAF
    • MCP-1
    • MCP1
    • MGC9434
    • monocyte chemoattractant protein 1
    • monocyte chemoattractant protein-1
    • monocyte chemotactic and activating factor
    • monocyte chemotactic protein 1
    • monocyte secretory protein JE
    • SCYA2
    • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
    • small inducible cytokine subfamily A (Cys-Cys), member 2
    • small-inducible cytokine A2
    • SMC-CF
  • NCBI_Gene: 6347
  • UniProtKB: P13500

Human CCL2 Predicted Functions

Predicted to enable CCR2 chemokine receptor binding activity and chemokine activity. Involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and regulation of apoptotic process. Located in extracellular region. Implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Biomarker of several diseases, including autoimmune disease (multiple); liver disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and retinal disease (multiple).

Mouse Ccl2 Target Information

Mouse Ccl2 Predicted Functions

Enables CCR2 chemokine receptor binding activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of leukocyte chemotaxis; and positive regulation of macromolecule metabolic process. Acts upstream of or within response to bacterium and response to wounding. Located in extracellular space. Is expressed in several structures, including heart; intestine smooth muscle circular layer; nervous system; retina; and vibrissa. Used to study age related macular degeneration. Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

Rat Ccl2 Target Information

  • Target Symbol: Ccl2, C-C motif chemokine ligand 2
  • Alias:
    • C-C motif chemokine 2
    • chemokine (C-C motif) ligand 2
    • immediate-early serum-responsive JE protein
    • immediate-early serum-responsive protein JE
    • MCP-1
    • monocyte chemoattractant protein 1
    • monocyte chemoattractant protein-1
    • monocyte chemotactic protein 1
    • Scya2
    • Sigje
    • small inducible cytokine A2
    • Small inducible gene JE
    • small-inducible cytokine A2
  • NCBI_Gene: 24770
  • UniProtKB: P14844

Rat Ccl2 Predicted Functions

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and leukocyte migration. Located in several cellular components, including perikaryon; perinuclear region of cytoplasm; and rough endoplasmic reticulum. Colocalizes with C-fiber. Used to study several diseases, including artery disease (multiple); diabetic angiopathy; interstitial lung disease (multiple); kidney disease (multiple); and pancreatitis (multiple). Biomarker of several diseases, including artery disease (multiple); cerebrovascular disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

CCL2 Target News

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    Glucokinase Inactivation Ameliorates Lipid Accumulation and Exerts Favorable Effects on Lipid Metabolism in Hepatocytes
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    3.747
    Hexokinase 2-mediated glycolysis supports inflammatory responses to Porphyromonas gingivalis in gingival fibroblasts
    BMC Oral Health
    9
    ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury
    Cell Death & Disease
    5.6
    LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation
    INTERNATIONAL IMMUNOPHARMACOLOGY
    5.714
    Acteoside alleviates asthma by modulating ROS-responsive NF-κB/MAPK signaling pathway
    INTERNATIONAL IMMUNOPHARMACOLOGY
    8.101
    Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells
    FREE RADICAL BIOLOGY AND MEDICINE
    10.723
    Multifunctional chondroitin sulfate based hydrogels for promoting infected diabetic wounds healing by chemo-photothermal antibacterial and cytokine modulation
    CARBOHYDRATE POLYMERS
    9.7
    CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment
    CANCER LETTERS
    7.5
    Oxoaporphine Pr(III) complex inhibits hepatocellular carcinoma progression and metastasis by disrupting tumor cell–macrophage crosstalk
    BIOMEDICINE & PHARMACOTHERAPY
    5.8
    Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes
    BIOCHEMICAL PHARMACOLOGY
    5.8
    BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
    3.3
    Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway
    Journal of Natural Medicines
    3.6
    Angiotensin II type 2 receptor agonist attenuates LPS-induced acute lung injury through modulating THP-1-derived macrophage reprogramming
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
    6.388
    Silibinin attenuated pseudo-allergic reactions and mast cell degranulation via PLCγ and PI3K/Akt signaling pathway
    PHYTOTHERAPY RESEARCH
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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