Mouse CCL2/MCP-1 ELISA Kit

$350.00$450.00

TARGETS CCL2
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 20.54 pg/mL
Range 156.25 pg/mL – 10000 pg/mL
Assay Time 3.5 h
Recovery 89% – 113%
Average Recovery 1
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 20.54 pg/mL
Range 156.25 pg/mL – 10000 pg/mL
Assay Time 3.5 h
Recovery 89% – 113%
Average Recovery 1
Intra Precision 3.3% – 4.6%
Inter-Precision 3.8% – 4.1%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL2/MCP-1 monoclonal antibody
Mouse CCL2/MCP-1 freeze-dried standard
CCL2/MCP-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse CCL2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL2 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL2

CCL2 Target Infomation Overview

  • Target Symbol: CCL2, C-C motif chemokine ligand 2
  • Gene Groups: Chemokine ligands
  • Alias: MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
  • Previous Names: SCYA2
  • Alias Names: monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2, C-C motif chemokine ligand 2 Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human CCL2 Target Information

  • Target Symbol: CCL2, C-C motif chemokine ligand 2
  • Alias:
    • C-C motif chemokine 2
    • chemokine (C-C motif) ligand 2
    • GDCF-2
    • HC11
    • HSMCR30
    • MCAF
    • MCP-1
    • MCP1
    • MGC9434
    • monocyte chemoattractant protein 1
    • monocyte chemoattractant protein-1
    • monocyte chemotactic and activating factor
    • monocyte chemotactic protein 1
    • monocyte secretory protein JE
    • SCYA2
    • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
    • small inducible cytokine subfamily A (Cys-Cys), member 2
    • small-inducible cytokine A2
    • SMC-CF
  • NCBI_Gene: 6347
  • UniProtKB: P13500

Human CCL2 Predicted Functions

Predicted to enable CCR2 chemokine receptor binding activity and chemokine activity. Involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and regulation of apoptotic process. Located in extracellular region. Implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Biomarker of several diseases, including autoimmune disease (multiple); liver disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and retinal disease (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Ccl2 Target Information

Mouse Ccl2 Predicted Functions

Enables CCR2 chemokine receptor binding activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of leukocyte chemotaxis; and positive regulation of macromolecule metabolic process. Acts upstream of or within response to bacterium and response to wounding. Located in extracellular space. Is expressed in several structures, including heart; intestine smooth muscle circular layer; nervous system; retina; and vibrissa. Used to study age related macular degeneration. Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Ccl2 Target Information

  • Target Symbol: Ccl2, C-C motif chemokine ligand 2
  • Alias:
    • C-C motif chemokine 2
    • chemokine (C-C motif) ligand 2
    • immediate-early serum-responsive JE protein
    • immediate-early serum-responsive protein JE
    • MCP-1
    • monocyte chemoattractant protein 1
    • monocyte chemoattractant protein-1
    • monocyte chemotactic protein 1
    • Scya2
    • Sigje
    • small inducible cytokine A2
    • Small inducible gene JE
    • small-inducible cytokine A2
  • NCBI_Gene: 24770
  • UniProtKB: P14844

Rat Ccl2 Predicted Functions

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and leukocyte migration. Located in several cellular components, including perikaryon; perinuclear region of cytoplasm; and rough endoplasmic reticulum. Colocalizes with C-fiber. Used to study several diseases, including artery disease (multiple); diabetic angiopathy; interstitial lung disease (multiple); kidney disease (multiple); and pancreatitis (multiple). Biomarker of several diseases, including artery disease (multiple); cerebrovascular disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

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CCL2 Target News

[catlist tags=”ccl2″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

Telotristat Etiprate alleviates rheumatoid arthritis by targeting LGALS3 and affecting MAPK signaling
3.4
TIFA contributes to periodontitis in diabetic mice via activating the NF‑κB signaling pathway
Molecular Medicine Reports
5.9
Alanyl-Glutamine Dipeptide Attenuates Non-Alcoholic Fatty Liver Disease Induced by a High-Fat Diet in Mice by Improving Gut Microbiota Dysbiosis
Nutrients
6.706
Exogenous Nucleotides Ameliorate Ageing-Related Intestinal Inflammation in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice
Nutrients
14.978
Hypoxia-Inducible Factor 2α Attenuates Renal Ischemia-Reperfusion Injury by Suppressing CD36-Mediated Lipid Accumulation in Dendritic Cells in a Mouse Model
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
1.798
Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation
EXPERIMENTAL ANIMALS
8.3
Regulator of G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell self-renewal in mice
CELLULAR & MOLECULAR BIOLOGY LETTERS
2.65
Prevention of Bleomycin-Induced Pulmonary Inflammation and Fibrosis in Mice by Bilobalide
Evidence-based Complementary and Alternative Medicine
5.5
Decreased miR-17-92 cluster correlates with senescence features, disrupted oxidative homeostasis, and impaired therapeutic efficacy of mesenchymal stem cells
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
3
Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo
Chemical Biology & Drug Design
19.865
TREM2hi resident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis
Nature Metabolism
9
ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury
Cell Death & Disease
17.1
Nanoscaled Titanium Oxide Layer Provokes Quick Osseointegration on 3D-Printed Dental Implants: A Domino Effect Induced by Hydrophilic Surface
ACS Nano
10.383
Engineered Apoptosis-Bioinspired Nanoparticles Initiate Immune Cascade for Cancer Immunotherapy of Malignant Ascites
ACS Applied Materials & Interfaces
8.039
Discovery of the First-in-Class Intestinal Restricted FXR and FABP1 Dual Modulator ZLY28 for the Treatment of Nonalcoholic Fatty Liver Disease
JOURNAL OF MEDICINAL CHEMISTRY
7.046
Constraint-induced movement therapy alleviates motor impairment by inhibiting the accumulation of neutrophil extracellular traps in ischemic cortex
NEUROBIOLOGY OF DISEASE
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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