Name: EasyGo!™ Human CCL2/MCP-1 One-Step ELISA Kit
SKU: EK187EG
Availability: InStock

EK187EG

EasyGo!™ Human CCL2/MCP-1 One-Step ELISA Kit

$349.00 – $499.00

Related Target	CCL2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20uL
Sensitivity	0.36pg/ml
Array Range	3.91-250pg/ml
Assay Time	90min
Recovery	70%-130%
Average Recovery	/

Size

Clear

SKU: EK187EG Category: EasyGo! One-Step ELISA Kit

ELISA Kit Detail Information

Related Target	CCL2
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20uL
Sensitivity	0.36pg/ml
Array Range	3.91-250pg/ml
Assay Time	90min
Recovery	70%-130%
Average Recovery	/
Intra Precision	< 10%
Inter Precision	< 10%
Plate	Detachable 96-well Plate
Storage	2-8℃
Delivery	blue ice
Components	Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×)
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

CCL2

CCL2 Target Infomation Overview

Target Symbol: CCL2, C-C motif chemokine ligand 2
Gene Groups: Chemokine ligands
Alias: MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
Previous Names: SCYA2
Alias Names: monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2, C-C motif chemokine ligand 2 Target Infomation by Species

Human
Mouse
Rat

Human CCL2 Target Information

Target Symbol: CCL2, C-C motif chemokine ligand 2
Alias:
- C-C motif chemokine 2
- chemokine (C-C motif) ligand 2
- GDCF-2
- HC11
- HSMCR30
- MCAF
- MCP-1
- MCP1
- MGC9434
- monocyte chemoattractant protein 1
- monocyte chemoattractant protein-1
- monocyte chemotactic and activating factor
- monocyte chemotactic protein 1
- monocyte secretory protein JE
- SCYA2
- small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
- small inducible cytokine subfamily A (Cys-Cys), member 2
- small-inducible cytokine A2
- SMC-CF
NCBI_Gene: 6347
UniProtKB: P13500

Human CCL2 Predicted Functions

Predicted to enable CCR2 chemokine receptor binding activity and chemokine activity. Involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and regulation of apoptotic process. Located in extracellular region. Implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Biomarker of several diseases, including autoimmune disease (multiple); liver disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and retinal disease (multiple).

Mouse Ccl2 Target Information

Target Symbol: Ccl2, chemokine (C-C motif) ligand 2
Alias:
- AI323594
- expressed sequence AI323594
- HC11
- MCAF
- MCP-1
- MCP1
- monocyte chemoattractant protein-1
- monocyte chemotactic protein
- Scya2
- Sigje
- small inducible cytokine A2
- small inducible gene JE
- SMC-CF
NCBI_Gene: 20296

Mouse Ccl2 Predicted Functions

Enables CCR2 chemokine receptor binding activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of leukocyte chemotaxis; and positive regulation of macromolecule metabolic process. Acts upstream of or within response to bacterium and response to wounding. Located in extracellular space. Is expressed in several structures, including heart; intestine smooth muscle circular layer; nervous system; retina; and vibrissa. Used to study age related macular degeneration. Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

Rat Ccl2 Target Information

Target Symbol: Ccl2, C-C motif chemokine ligand 2
Alias:
- C-C motif chemokine 2
- chemokine (C-C motif) ligand 2
- immediate-early serum-responsive JE protein
- immediate-early serum-responsive protein JE
- MCP-1
- monocyte chemoattractant protein 1
- monocyte chemoattractant protein-1
- monocyte chemotactic protein 1
- Scya2
- Sigje
- small inducible cytokine A2
- Small inducible gene JE
- small-inducible cytokine A2
NCBI_Gene: 24770
UniProtKB: P14844

Rat Ccl2 Predicted Functions

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and leukocyte migration. Located in several cellular components, including perikaryon; perinuclear region of cytoplasm; and rough endoplasmic reticulum. Colocalizes with C-fiber. Used to study several diseases, including artery disease (multiple); diabetic angiopathy; interstitial lung disease (multiple); kidney disease (multiple); and pancreatitis (multiple). Biomarker of several diseases, including artery disease (multiple); cerebrovascular disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

Competitive ELISA: Principles, Methods, and Key Differences

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ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

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