Name: EasyGo!&trade; Human VEGF One-Step ELISA Kit
SKU: EK183EGA
Availability: InStock

EK183EGA

EasyGo!™ Human VEGF One-Step ELISA Kit

$294.00 – $420.00

Related Target	VEGFA
Species	human
Sample Type	Serum, plasma
Sample Volume	Serum, plasma: 20 μL
Sensitivity	3.03 pg/mL
Array Range	31.25 pg/mL - 2000 pg/mL
Assay Time	1.5h
Recovery	85% - 112%
Average Recovery	94%

ELISA Kit Detail Information

Related Target	VEGFA
Species	human
Sample Type	Serum, plasma
Sample Volume	Serum, plasma: 20 μL
Sensitivity	3.03 pg/mL
Array Range	31.25 pg/mL - 2000 pg/mL
Assay Time	1.5h
Recovery	85% - 112%
Average Recovery	94%
Intra Precision	4.1% - 6.4%
Inter Precision	2.1% - 2.6%
Plate	Detachable 96-well Plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ Blue Ice Transport
Components	1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Standard Microplate Wells
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human VEGF has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for VEGF (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then VEGF present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of VEGF bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

VEGFA

VEGFA Target Infomation Overview

Target Symbol: VEGFA, vascular endothelial growth factor A
Gene Groups: VEGF family
Alias: VEGF-A; VPF
Previous Names: VEGF
Alias Names: vascular endothelial growth factor

VEGFA, vascular endothelial growth factor A Target Infomation by Species

Human
Mouse
Rat

Human VEGFA Target Information

Target Symbol: VEGFA, vascular endothelial growth factor A
Alias:
- MGC70609
- MVCD1
- vascular endothelial growth factor
- vascular endothelial growth factor A121
- vascular endothelial growth factor A165
- vascular permeability factor
- VEGF
- VEGF-A
- VPF
NCBI_Gene: 7422
UniProtKB: P15692

Human VEGFA Predicted Functions

Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).

Mouse Vegfa Target Information

Target Symbol: Vegfa, vascular endothelial growth factor A
Alias:
- vascular endothelial growth factor
- Vegf
- VEGF-A
- VEGF120
- VEGF164
- VEGF188
- VPF
NCBI_Gene: 22339

Mouse Vegfa Predicted Functions

Enables extracellular matrix binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including generation of neurons; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and vasculature development. Located in cytoplasm; extracellular space; and nucleus. Is expressed in several structures, including alimentary system; brain; egg cylinder; genitourinary system; and heart. Used to study DiGeorge syndrome; amyotrophic lateral sclerosis type 1; newborn respiratory distress syndrome; and psoriasis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

Rat Vegfa Target Information

Target Symbol: Vegfa, vascular endothelial growth factor A
Alias:
- vascular endothelial growth factor
- vascular permeability factor
- Vegf
- VEGF-A
- VEGF111
- VEGF164
- VPF
NCBI_Gene: 83785

Rat Vegfa Predicted Functions

Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

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