Name: EasyGo!&trade; Human IL-2 One-Step ELISA Kit
SKU: EK102EGA
Availability: InStock

EK102EGA

EasyGo!™ Human IL-2 One-Step ELISA Kit

$294.00 – $420.00

Related Target	IL2
Species	human
Sample Type	Serum, plasma
Sample Volume	Serum, plasma:10μL
Sensitivity	1.10 pg/mL
Array Range	1.56 pg/mL - 100 pg/mL
Assay Time	1.5h
Recovery	79% - 121%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	IL2
Species	human
Sample Type	Serum, plasma
Sample Volume	Serum, plasma:10μL
Sensitivity	1.10 pg/mL
Array Range	1.56 pg/mL - 100 pg/mL
Assay Time	1.5h
Recovery	79% - 121%
Average Recovery	102%
Intra Precision	5.4% - 7.5%
Inter Precision	4.5% - 6.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Standard Microplate Wells
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IL-2 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-2 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-2 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-2 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

IL2

IL2 Target Infomation Overview

Target Symbol: IL2, interleukin 2
Gene Groups: Interleukins
Alias: IL-2; TCGF
Alias Names: T cell growth factor

IL2, interleukin 2 Target Infomation by Species

Human
Mouse
Rat

Human IL2 Target Information

Target Symbol: IL2, interleukin 2
Alias:
- aldesleukin
- IL-2
- interleukin-2
- involved in regulation of T-cell clonal expansion
- lymphokine
- T cell growth factor
- TCGF
NCBI_Gene: 3558
UniProtKB: P60568

Human IL2 Predicted Functions

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in leukocyte activation involved in immune response; positive regulation of interleukin-17 production; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within negative regulation of B cell apoptotic process; positive regulation of B cell proliferation; and positive regulation of activated T cell proliferation. Predicted to be located in extracellular space. Implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Zellweger syndrome; aggressive periodontitis; anogenital venereal wart; autoimmune disease (multiple); and uveitis (multiple).

Mouse Il2 Target Information

Mouse Il2 Predicted Functions

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren’s syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).

Rat Il2 Target Information

Target Symbol: Il2, interleukin 2
Alias:
- IL-2
- interleukin-2
- T-cell growth factor
- TCGF
NCBI_Gene: 116562
UniProtKB: P17108

Rat Il2 Predicted Functions

Enables carbohydrate binding activity; glycosphingolipid binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of T cell activation; protein kinase C-activating G protein-coupled receptor signaling pathway; and regulation of protein phosphorylation. Located in extracellular space. Used to study cystitis. Biomarker of allergic rhinitis; colorectal carcinoma; and tongue squamous cell carcinoma. Human ortholog(s) of this gene implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).

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