EasyGo!™ Human IL-2 One-Step ELISA Kit
$349.00 – $499.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma |
| Sample Volume | Serum, plasma:10μL |
| Sensitivity | 1.10 pg/mL |
| Array Range | 1.56 pg/mL - 100 pg/mL |
| Assay Time | 1.5h |
| Recovery | 79% - 121% |
| Average Recovery | 102% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma |
| Sample Volume | Serum, plasma:10μL |
| Sensitivity | 1.10 pg/mL |
| Array Range | 1.56 pg/mL - 100 pg/mL |
| Assay Time | 1.5h |
| Recovery | 79% - 121% |
| Average Recovery | 102% |
| Intra Precision | 5.4% - 7.5% |
| Inter Precision | 4.5% - 6.0% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Standard Microplate Wells |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IL-2 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-2 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-2 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-2 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
IL2
IL2 Target Infomation Overview
- Target Symbol: IL2, interleukin 2
- Gene Groups: Interleukins
- Alias: IL-2; TCGF
- Alias Names: T cell growth factor
IL2, interleukin 2 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL2 Target Information
- Target Symbol: IL2, interleukin 2
- Alias:
- aldesleukin
- IL-2
- interleukin-2
- involved in regulation of T-cell clonal expansion
- lymphokine
- T cell growth factor
- TCGF
- NCBI_Gene: 3558
- UniProtKB: P60568
Human IL2 Predicted Functions
Enables cytokine activity and interleukin-2 receptor binding activity. Involved in leukocyte activation involved in immune response; positive regulation of interleukin-17 production; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within negative regulation of B cell apoptotic process; positive regulation of B cell proliferation; and positive regulation of activated T cell proliferation. Predicted to be located in extracellular space. Implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Zellweger syndrome; aggressive periodontitis; anogenital venereal wart; autoimmune disease (multiple); and uveitis (multiple).
Mouse Il2 Target Information
- Target Symbol: Il2, interleukin 2
- Alias:
- IL-2
- Il-2
- NCBI_Gene: 16183
Mouse Il2 Predicted Functions
Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren’s syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).
Rat Il2 Target Information
- Target Symbol: Il2, interleukin 2
- Alias:
- IL-2
- interleukin-2
- T-cell growth factor
- TCGF
- NCBI_Gene: 116562
- UniProtKB: P17108
Rat Il2 Predicted Functions
Enables carbohydrate binding activity; glycosphingolipid binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of T cell activation; protein kinase C-activating G protein-coupled receptor signaling pathway; and regulation of protein phosphorylation. Located in extracellular space. Used to study cystitis. Biomarker of allergic rhinitis; colorectal carcinoma; and tongue squamous cell carcinoma. Human ortholog(s) of this gene implicated in several diseases, including Takayasu’s arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).
Products with the Same Target - IL2
Citations for EasyGo!™ Human IL-2 One-Step ELISA Kit
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Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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