Human MIF ELISA Kit

$350.00$450.00

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TARGETSMIF
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity1.00 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery95% – 112%
Average Recovery1.05
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity1.00 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery95% – 112%
Average Recovery1.05
Intra Precision2.0% – 4.0%
Inter-Precision2.0% – 4.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MIF monoclonal antibody
Human MIF freeze-dried standard
MIF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MIF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MIF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MIF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MIF

MIF Target Infomation Overview

  • Target Symbol: MIF, macrophage migration inhibitory factor
  • Alias: GIF
  • Previous Names: GLIF
  • Alias Names: glycosylation-inhibiting factor; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor)

MIF, macrophage migration inhibitory factor Target Infomation by Species

HumanMouseRat

Human MIF Target Information

  • Target Symbol: MIF, macrophage migration inhibitory factor
  • Alias:
    • GIF
    • GLIF
    • L-dopachrome isomerase
    • L-dopachrome tautomerase
    • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
    • MMIF
    • phenylpyruvate tautomerase
  • NCBI_Gene: 4282
  • UniProtKB: P14174

Human MIF Predicted Functions

Enables several functions, including identical protein binding activity; intramolecular oxidoreductase activity; and receptor ligand activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of protein phosphorylation; and regulation of intracellular signal transduction. Located in several cellular components, including cell surface; extracellular space; and nucleoplasm. Implicated in allergic disease; asthma; cystic fibrosis; lung disease (multiple); and obesity. Biomarker of artery disease (multiple); asthma; lung disease (multiple); obesity; and rhinitis.

Mouse Mif Target Information

Mouse Mif Predicted Functions

Enables cytokine activity; phenylpyruvate tautomerase activity; and protease binding activity. Involved in several processes, including negative regulation of apoptotic process; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within DNA damage response, signal transduction by p53 class mediator; cell aging; and regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in allergic disease; asthma; cystic fibrosis; lung disease (multiple); and obesity. Orthologous to human MIF (macrophage migration inhibitory factor).

Rat Mif Target Information

  • Target Symbol: Mif, macrophage migration inhibitory factor
  • Alias:
    • glutathione-binding 13 kDa protein
    • L-dopachrome isomerase
    • L-dopachrome tautomerase
    • LOC103694877
    • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
    • MGC72801
    • phenylpyruvate tautomerase
  • NCBI_Gene: 81683

Rat Mif Predicted Functions

Predicted to enable several functions, including identical protein binding activity; intramolecular oxidoreductase activity; and receptor ligand activity. Involved in several processes, including positive regulation of transport; response to steroid hormone; and response to vitamin. Located in cytoplasm; extracellular space; and nucleus. Used to study several diseases, including acute necrotizing pancreatitis; cardiomyopathy (multiple); lung disease (multiple); retinitis; and toxic shock syndrome. Biomarker of borna disease; cystitis; kidney disease; myocardial infarction; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in allergic disease; asthma; cystic fibrosis; lung disease (multiple); and obesity. Orthologous to human MIF (macrophage migration inhibitory factor).

MIF Target News

    12.153
    MAPK4 silencing in gastric cancer drives liver metastasis by positive feedback between cancer cells and macrophages
    EXPERIMENTAL AND MOLECULAR MEDICINE

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