Rat sICAM-1/CD54 ELISA Kit

$350.00$450.00

TARGETS ICAM1
SPECIES Rat
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL
Sensitivity 0.48 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 73% – 90%
Average Recovery 0.83
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 100 μL
Sensitivity 0.48 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 73% – 90%
Average Recovery 0.83
Intra Precision 3.8% – 4.2%
Inter-Precision 2.7% – 3.4%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sICAM-1/CD54 monoclonal antibody
Rat sICAM-1/CD54 freeze-dried standard
sICAM-1/CD54 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat sICAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sICAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sICAM-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

ICAM1

ICAM1 Target Infomation Overview

  • Target Symbol: ICAM1, intercellular adhesion molecule 1
  • Gene Groups: CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
  • Alias: BB2; CD54
  • Alias Names: human rhinovirus receptor

ICAM1, intercellular adhesion molecule 1 Target Infomation by Species

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Human ICAM1 Target Information

  • Target Symbol: ICAM1, intercellular adhesion molecule 1
  • Alias:
    • BB2
    • CD54
    • cell surface glycoprotein P3.58
    • human rhinovirus receptor
    • ICAM-1
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
    • major group rhinovirus receptor
    • P3.58
  • NCBI_Gene: 3383
  • UniProtKB: P05362

Human ICAM1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; cellular response to amyloid-beta; and negative regulation of apoptotic process. Located in extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetes mellitus (multiple); hematologic cancer (multiple); and lung disease (multiple).

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Mouse Icam1 Target Information

Mouse Icam1 Predicted Functions

Predicted to enable integrin binding activity. Acts upstream of or within several processes, including T cell antigen processing and presentation; cellular response to glucose stimulus; and cellular response to leukemia inhibitory factor. Located in external side of plasma membrane and immunological synapse. Colocalizes with membrane raft. Is expressed in several structures, including cardiovascular system; liver vasculature; lung; skin; and thymus primordium. Used to study malaria. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; inflammatory bowel disease (multiple); liver cirrhosis; and uveitis (multiple). Orthologous to human ICAM1 (intercellular adhesion molecule 1).

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Rat Icam1 Target Information

Rat Icam1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and gonad development. Located in cell surface; extracellular space; and plasma membrane. Used to study diabetic angiopathy; mesenteric vascular occlusion; nephritis; pre-eclampsia; and uveitis. Biomarker of several diseases, including cerebrovascular disease (multiple); colitis (multiple); kidney failure (multiple); lung disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Orthologous to human ICAM1 (intercellular adhesion molecule 1).

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ICAM1 Target News

[catlist tags=”icam1″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

2.4
Electroacupuncture treatment improves postoperative ileus by inhibiting the Th1 cell-mediated inflammatory response through the vagus nerve
Acupuncture in Medicine
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Multiple Sclerosis
Sepsis
Systemic Lupus Erythematosus

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