Mouse sICAM-1/CD54 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	ICAM1
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	1.27 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	89% – 112%
Average Recovery	1.02

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	1.27 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	89% – 112%
Average Recovery	1.02
Intra Precision	3.6% – 4.0%
Inter-Precision	4.1% – 5.1%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sICAM-1/CD54 monoclonal antibody Mouse sICAM-1/CD54 freeze-dried standard sICAM-1/CD54 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse sICAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sICAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sICAM-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

ICAM1

ICAM1 Target Infomation Overview

Target Symbol: ICAM1, intercellular adhesion molecule 1
Gene Groups: CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
Alias: BB2; CD54
Alias Names: human rhinovirus receptor

ICAM1, intercellular adhesion molecule 1 Target Infomation by Species

HumanMouseRat

Human ICAM1 Target Information

Target Symbol: ICAM1, intercellular adhesion molecule 1
Alias:
- BB2
- CD54
- cell surface glycoprotein P3.58
- human rhinovirus receptor
- ICAM-1
- intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
- major group rhinovirus receptor
- P3.58
NCBI_Gene: 3383
UniProtKB: P05362

Human ICAM1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; cellular response to amyloid-beta; and negative regulation of apoptotic process. Located in extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetes mellitus (multiple); hematologic cancer (multiple); and lung disease (multiple).

Mouse Icam1 Target Information

Target Symbol: Icam1, intercellular adhesion molecule 1
Alias:
- CD54
- Icam-1
- Ly-47
- lymphocyte antigen 47
- MALA-2
- MGC:6195
NCBI_Gene: 15894

Mouse Icam1 Predicted Functions

Predicted to enable integrin binding activity. Acts upstream of or within several processes, including T cell antigen processing and presentation; cellular response to glucose stimulus; and cellular response to leukemia inhibitory factor. Located in external side of plasma membrane and immunological synapse. Colocalizes with membrane raft. Is expressed in several structures, including cardiovascular system; liver vasculature; lung; skin; and thymus primordium. Used to study malaria. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; inflammatory bowel disease (multiple); liver cirrhosis; and uveitis (multiple). Orthologous to human ICAM1 (intercellular adhesion molecule 1).

Rat Icam1 Target Information

Target Symbol: Icam1, intercellular adhesion molecule 1
Alias:
- CD54
- ICAM
- ICAM-1
NCBI_Gene: 25464
UniProtKB: Q00238

Rat Icam1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and gonad development. Located in cell surface; extracellular space; and plasma membrane. Used to study diabetic angiopathy; mesenteric vascular occlusion; nephritis; pre-eclampsia; and uveitis. Biomarker of several diseases, including cerebrovascular disease (multiple); colitis (multiple); kidney failure (multiple); lung disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Orthologous to human ICAM1 (intercellular adhesion molecule 1).