Human sICAM-1/CD54 ELISA Kit

$350.00$450.00

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TARGETSICAM1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.81 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery99% – 118%
Average Recovery1.1
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.81 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery99% – 118%
Average Recovery1.1
Intra Precision4.9%-6.7%
Inter-Precision6.3% – 7.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sICAM-1/CD54 monoclonal antibody
Human sICAM-1/CD54 freeze-dried standard
sICAM-1/CD54 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sICAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sICAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sICAM-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

ICAM1

ICAM1 Target Infomation Overview

  • Target Symbol: ICAM1, intercellular adhesion molecule 1
  • Gene Groups: CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
  • Alias: BB2; CD54
  • Alias Names: human rhinovirus receptor

ICAM1, intercellular adhesion molecule 1 Target Infomation by Species

HumanMouseRat

Human ICAM1 Target Information

  • Target Symbol: ICAM1, intercellular adhesion molecule 1
  • Alias:
    • BB2
    • CD54
    • cell surface glycoprotein P3.58
    • human rhinovirus receptor
    • ICAM-1
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
    • major group rhinovirus receptor
    • P3.58
  • NCBI_Gene: 3383
  • UniProtKB: P05362

Human ICAM1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; cellular response to amyloid-beta; and negative regulation of apoptotic process. Located in extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetes mellitus (multiple); hematologic cancer (multiple); and lung disease (multiple).

Mouse Icam1 Target Information

Mouse Icam1 Predicted Functions

Predicted to enable integrin binding activity. Acts upstream of or within several processes, including T cell antigen processing and presentation; cellular response to glucose stimulus; and cellular response to leukemia inhibitory factor. Located in external side of plasma membrane and immunological synapse. Colocalizes with membrane raft. Is expressed in several structures, including cardiovascular system; liver vasculature; lung; skin; and thymus primordium. Used to study malaria. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; inflammatory bowel disease (multiple); liver cirrhosis; and uveitis (multiple). Orthologous to human ICAM1 (intercellular adhesion molecule 1).

Rat Icam1 Target Information

Rat Icam1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and gonad development. Located in cell surface; extracellular space; and plasma membrane. Used to study diabetic angiopathy; mesenteric vascular occlusion; nephritis; pre-eclampsia; and uveitis. Biomarker of several diseases, including cerebrovascular disease (multiple); colitis (multiple); kidney failure (multiple); lung disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Orthologous to human ICAM1 (intercellular adhesion molecule 1).

ICAM1 Target News

    4.004
    5-epi-ent-Kaurane diterpenoids from the aerial parts of Isodon eriocalyx and their anti-atherosclerotic potential
    PHYTOCHEMISTRY
    5.714
    Acteoside alleviates asthma by modulating ROS-responsive NF-κB/MAPK signaling pathway
    INTERNATIONAL IMMUNOPHARMACOLOGY
    4.6
    Long-term abuse of caffeine sodium benzoate induces endothelial cells injury and leads to coagulation dysfunction
    IUBMB LIFE
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Sepsis
    Systemic Lupus Erythematosus

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