Mouse sICAM-1/CD54 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 1.27 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 89% – 112% |
| Average Recovery | 102% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 1.27 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 89% – 112% |
| Average Recovery | 102% |
| Intra Precision | 3.6% – 4.0% |
| Inter Precision | 4.1% – 5.1% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sICAM-1/CD54 monoclonal antibody Mouse sICAM-1/CD54 freeze-dried standard sICAM-1/CD54 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse sICAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sICAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sICAM-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
ICAM1
ICAM1 Target Infomation Overview
- Target Symbol: ICAM1, intercellular adhesion molecule 1
- Gene Groups: CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
- Alias: BB2; CD54
- Alias Names: human rhinovirus receptor
ICAM1, intercellular adhesion molecule 1 Target Infomation by Species
- Human
- Mouse
- Rat
Human ICAM1 Target Information
- Target Symbol: ICAM1, intercellular adhesion molecule 1
- Alias:
- BB2
- CD54
- cell surface glycoprotein P3.58
- human rhinovirus receptor
- ICAM-1
- intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
- major group rhinovirus receptor
- P3.58
- NCBI_Gene: 3383
- UniProtKB: P05362
Human ICAM1 Predicted Functions
Enables integrin binding activity. Involved in several processes, including T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; cellular response to amyloid-beta; and negative regulation of apoptotic process. Located in extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetes mellitus (multiple); hematologic cancer (multiple); and lung disease (multiple).
Mouse Icam1 Target Information
- Target Symbol: Icam1, intercellular adhesion molecule 1
- Alias:
- CD54
- Icam-1
- Ly-47
- lymphocyte antigen 47
- MALA-2
- MGC:6195
- NCBI_Gene: 15894
Mouse Icam1 Predicted Functions
Predicted to enable integrin binding activity. Acts upstream of or within several processes, including T cell antigen processing and presentation; cellular response to glucose stimulus; and cellular response to leukemia inhibitory factor. Located in external side of plasma membrane and immunological synapse. Colocalizes with membrane raft. Is expressed in several structures, including cardiovascular system; liver vasculature; lung; skin; and thymus primordium. Used to study malaria. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; inflammatory bowel disease (multiple); liver cirrhosis; and uveitis (multiple). Orthologous to human ICAM1 (intercellular adhesion molecule 1).
Rat Icam1 Target Information
- Target Symbol: Icam1, intercellular adhesion molecule 1
- Alias:
- CD54
- ICAM
- ICAM-1
- NCBI_Gene: 25464
- UniProtKB: Q00238
Rat Icam1 Predicted Functions
Enables integrin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and gonad development. Located in cell surface; extracellular space; and plasma membrane. Used to study diabetic angiopathy; mesenteric vascular occlusion; nephritis; pre-eclampsia; and uveitis. Biomarker of several diseases, including cerebrovascular disease (multiple); colitis (multiple); kidney failure (multiple); lung disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Orthologous to human ICAM1 (intercellular adhesion molecule 1).
Products with the Same Target - ICAM1
Citations for Mouse sICAM-1/CD54 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK1272
- ELISA Kit
Human B7-1/CD80 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1218
- ELISA Kit
Human Apolipoprotein E/Apo E ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK9125
- ELISA Kit
Glucagon ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1120
- ELISA Kit
Human Activin A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK406
- ELISA Kit
Guinea Pig IL-6 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1260
- ELISA Kit
Human alpha-Galactosidase A/GLA ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.