Mouse sICAM-1/CD54 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 1.27 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 89% – 112% |
| Average Recovery | 102% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 1.27 pg/mL |
| Array Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 89% – 112% |
| Average Recovery | 102% |
| Intra Precision | 3.6% – 4.0% |
| Inter Precision | 4.1% – 5.1% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sICAM-1/CD54 monoclonal antibody Mouse sICAM-1/CD54 freeze-dried standard sICAM-1/CD54 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse sICAM-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sICAM-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sICAM-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
ICAM1
ICAM1 Target Infomation Overview
- Target Symbol: ICAM1, intercellular adhesion molecule 1
- Gene Groups: CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
- Alias: BB2; CD54
- Alias Names: human rhinovirus receptor
ICAM1, intercellular adhesion molecule 1 Target Infomation by Species
- Human
- Mouse
- Rat
Human ICAM1 Target Information
- Target Symbol: ICAM1, intercellular adhesion molecule 1
- Alias:
- BB2
- CD54
- cell surface glycoprotein P3.58
- human rhinovirus receptor
- ICAM-1
- intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
- major group rhinovirus receptor
- P3.58
- NCBI_Gene: 3383
- UniProtKB: P05362
Human ICAM1 Predicted Functions
Enables integrin binding activity. Involved in several processes, including T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; cellular response to amyloid-beta; and negative regulation of apoptotic process. Located in extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetes mellitus (multiple); hematologic cancer (multiple); and lung disease (multiple).
Mouse Icam1 Target Information
- Target Symbol: Icam1, intercellular adhesion molecule 1
- Alias:
- CD54
- Icam-1
- Ly-47
- lymphocyte antigen 47
- MALA-2
- MGC:6195
- NCBI_Gene: 15894
Mouse Icam1 Predicted Functions
Predicted to enable integrin binding activity. Acts upstream of or within several processes, including T cell antigen processing and presentation; cellular response to glucose stimulus; and cellular response to leukemia inhibitory factor. Located in external side of plasma membrane and immunological synapse. Colocalizes with membrane raft. Is expressed in several structures, including cardiovascular system; liver vasculature; lung; skin; and thymus primordium. Used to study malaria. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; inflammatory bowel disease (multiple); liver cirrhosis; and uveitis (multiple). Orthologous to human ICAM1 (intercellular adhesion molecule 1).
Rat Icam1 Target Information
- Target Symbol: Icam1, intercellular adhesion molecule 1
- Alias:
- CD54
- ICAM
- ICAM-1
- NCBI_Gene: 25464
- UniProtKB: Q00238
Rat Icam1 Predicted Functions
Enables integrin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and gonad development. Located in cell surface; extracellular space; and plasma membrane. Used to study diabetic angiopathy; mesenteric vascular occlusion; nephritis; pre-eclampsia; and uveitis. Biomarker of several diseases, including cerebrovascular disease (multiple); colitis (multiple); kidney failure (multiple); lung disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Orthologous to human ICAM1 (intercellular adhesion molecule 1).
Products with the Same Target - ICAM1
Citations for Mouse sICAM-1/CD54 ELISA Kit
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