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Human IL-17E/IL-25 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 2.39 pg/mL |
| Range | 93.75 pg/mL – 6000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 116% |
| Average Recovery | 0.96 |
| Intra Precision | 2.8% – 4.7% |
| Inter-Precision | 2.7% – 4.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17E monoclonal antibody Human IL-17E freeze-dried standard IL-17E detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-17E antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17E present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of IL-17E in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL25
IL25 Target Infomation Overview
- Target Symbol: IL25, interleukin 25
- Gene Groups: Interleukins
- Alias: IL-25; IL-17E
- Previous Names: IL17E
- Alias Names: interleukin 17E
IL25, interleukin 25 Target Infomation by Species
HumanMouseRat
Human IL25 Target Information
- Target Symbol: IL25, interleukin 25
- Alias:
- IL-17E
- IL-25
- IL17E
- interleukin 17E
- interleukin-17E
- interleukin-25
- NCBI_Gene: 64806
- UniProtKB: Q9H293
Human IL25 Predicted Functions
Predicted to enable interleukin-17E receptor binding activity. Predicted to be involved in inflammatory response to antigenic stimulus and signal transduction. Predicted to act upstream of or within several processes, including eosinophil differentiation; response to fungus; and response to nematode. Predicted to be located in extracellular region. Biomarker of atopic dermatitis and psoriasis.
Mouse Il25 Target Information
- Target Symbol: Il25, interleukin 25
- Alias:
- IL-17E
- Il17e
- interleukin 17E
- NCBI_Gene: 140806
Mouse Il25 Predicted Functions
Enables cytokine activity. Acts upstream of or within several processes, including eosinophil differentiation; response to fungus; and response to nematode. Predicted to be located in extracellular space. Orthologous to human IL25 (interleukin 25).
Rat Il25 Target Information
- Target Symbol: Il25, interleukin 25
- Alias:
- interleukin-25
- LOC501996
- RGD1561632
- similar to IL25
- NCBI_Gene: 501996
- UniProtKB: D3ZLB1
Rat Il25 Predicted Functions
Predicted to enable cytokine activity. Involved in inflammatory response to antigenic stimulus. Located in extracellular space. Biomarker of gastric ulcer. Orthologous to human IL25 (interleukin 25).
