Mouse IL-17E/IL-25 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL25
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.22 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	73% – 99%
Average Recovery	0.83

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	1.22 pg/mL
Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	73% – 99%
Average Recovery	0.83
Intra Precision	3.2% – 3.6%
Inter-Precision	3.8% – 4.5%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17E/IL-25 monoclonal antibody Mouse IL-17E/IL-25 freeze-dried standard IL-17E/IL-25 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-17E antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17E present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-17E in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL25

IL25 Target Infomation Overview

Target Symbol: IL25, interleukin 25
Gene Groups: Interleukins
Alias: IL-25; IL-17E
Previous Names: IL17E
Alias Names: interleukin 17E

IL25, interleukin 25 Target Infomation by Species

HumanMouseRat

Human IL25 Target Information

Target Symbol: IL25, interleukin 25
Alias:
- IL-17E
- IL-25
- IL17E
- interleukin 17E
- interleukin-17E
- interleukin-25
NCBI_Gene: 64806
UniProtKB: Q9H293

Human IL25 Predicted Functions

Predicted to enable interleukin-17E receptor binding activity. Predicted to be involved in inflammatory response to antigenic stimulus and signal transduction. Predicted to act upstream of or within several processes, including eosinophil differentiation; response to fungus; and response to nematode. Predicted to be located in extracellular region. Biomarker of atopic dermatitis and psoriasis.

Mouse Il25 Target Information

Target Symbol: Il25, interleukin 25
Alias:
- IL-17E
- Il17e
- interleukin 17E
NCBI_Gene: 140806
- UniprotKB: Q8VHC9
- UniprotKB: Q8VHH8

Mouse Il25 Predicted Functions

Enables cytokine activity. Acts upstream of or within several processes, including eosinophil differentiation; response to fungus; and response to nematode. Predicted to be located in extracellular space. Orthologous to human IL25 (interleukin 25).

Rat Il25 Target Information

Target Symbol: Il25, interleukin 25
Alias:
- interleukin-25
- LOC501996
- RGD1561632
- similar to IL25
NCBI_Gene: 501996
UniProtKB: D3ZLB1

Rat Il25 Predicted Functions

Predicted to enable cytokine activity. Involved in inflammatory response to antigenic stimulus. Located in extracellular space. Biomarker of gastric ulcer. Orthologous to human IL25 (interleukin 25).