Rat IL-4 ELISA Kit

$350.00$450.00

TARGETS IL4
SPECIES Rat
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.26 pg/mL
Range 15.63 pg/mL – 1000 pg/mL
Assay Time 3.5 h
Recovery 86% – 106%
Average Recovery 0.99
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.26 pg/mL
Range 15.63 pg/mL – 1000 pg/mL
Assay Time 3.5 h
Recovery 86% – 106%
Average Recovery 0.99
Intra Precision 2.1% – 2.7%
Inter-Precision 3.1% – 4.3%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-4 monoclonal antibody
Rat IL-4 freeze-dried standard
IL-4 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL4

IL4 Target Infomation Overview

  • Target Symbol: IL4, interleukin 4
  • Gene Groups: Interleukins
  • Alias: BSF1; IL-4; BCGF1; BCGF-1; MGC79402
  • Alias Names: B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4, interleukin 4 Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human IL4 Target Information

  • Target Symbol: IL4, interleukin 4
  • Alias:
    • B cell growth factor 1
    • B_cell stimulatory factor 1
    • B-cell stimulatory factor 1
    • BCGF-1
    • BCGF1
    • binetrakin
    • BSF-1
    • BSF1
    • IL-4
    • interleukin 4 variant 2
    • interleukin-4
    • lymphocyte stimulatory factor 1
    • MGC79402
    • pitrakinra
  • NCBI_Gene: 3565
  • UniProtKB: P05112

Human IL4 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cellular response to transforming growth factor beta stimulus; negative regulation of complement-dependent cytotoxicity; and positive regulation of macromolecule metabolic process. Acts upstream of or within positive regulation of cell migration and positive regulation of cell population proliferation. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Biomarker of several diseases, including allergic rhinitis; alopecia universalis; anogenital venereal wart; autoimmune disease (multiple); and liver disease (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Il4 Target Information

Mouse Il4 Predicted Functions

Enables cytokine activity. Involved in several processes, including innate immune response in mucosa; negative regulation of white fat cell proliferation; and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation; positive regulation of macromolecule metabolic process; and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain; colon; hemolymphoid system; liver; and placenta. Used to study Sjogren’s syndrome; atopic dermatitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Il4 Target Information

Rat Il4 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).

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IL4 Target News

[catlist tags=”il4″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

3.1
Lipidomic profiling of amniotic fluid reveals aberrant fetal lung development and fetal growth disrupted by lipid disorders during gestational asthma
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
4
Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota
Frontiers in Nutrition
2.9
Glycoprotein Non-metastatic Melanoma Protein B (GPNMB) Protects Against Neuroinflammation and Neuronal Loss in Pilocarpine-induced Epilepsy via the Regulation of Microglial Polarization
NEUROSCIENCE
6.1
M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats
Neural Regeneration Research
17.1
Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke
ACS Nano
5.7
Quantitative susceptibility mapping in rats with minimal hepatic encephalopathy: Does iron overload aggravate cognitive impairment by promoting neuroinflammation?
NEUROIMAGE
2.7
Inhibition of the MLCK/MLC2 pathway protects against intestinal heat stroke-induced injury in rats
JOURNAL OF THERMAL BIOLOGY
2.7
Roles of prokineticin 2 in electroconvulsive shock-induced memory impairment via regulation of phenotype polarization in astrocytes
BEHAVIOURAL BRAIN RESEARCH
5.2
Integrating Widely Targeted Lipidomics and Transcriptomics Unravels Aberrant Lipid Metabolism and Identifies Potential Biomarkers of Food Allergies in Rats
MOLECULAR NUTRITION & FOOD RESEARCH
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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