Rat IL-4 High Sensitivity ELISA Kit

High Sensitivity ELISA

$380.00 – $480.00

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Datasheet

SDS File

TARGETS	IL4
SPECIES	Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.20 pg/mL
Range	3.13 pg/mL – 200 pg/mL
Assay Time	3.5 h
Recovery	81% – 101%
Average Recovery	0.9

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.20 pg/mL
Range	3.13 pg/mL – 200 pg/mL
Assay Time	3.5 h
Recovery	81% – 101%
Average Recovery	0.9
Intra Precision	5.0% – 6.8%
Inter-Precision	2.7% – 7.5%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-4 monoclonal antibody Rat IL-4 freeze-dried standard IL-4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-4 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-4 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-4 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL4

IL4 Target Infomation Overview

Target Symbol: IL4, interleukin 4
Gene Groups: Interleukins
Alias: BSF1; IL-4; BCGF1; BCGF-1; MGC79402
Alias Names: B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4, interleukin 4 Target Infomation by Species

HumanMouseRat

Human IL4 Target Information

Target Symbol: IL4, interleukin 4
Alias:
- B cell growth factor 1
- B_cell stimulatory factor 1
- B-cell stimulatory factor 1
- BCGF-1
- BCGF1
- binetrakin
- BSF-1
- BSF1
- IL-4
- interleukin 4 variant 2
- interleukin-4
- lymphocyte stimulatory factor 1
- MGC79402
- pitrakinra
NCBI_Gene: 3565
UniProtKB: P05112

Human IL4 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cellular response to transforming growth factor beta stimulus; negative regulation of complement-dependent cytotoxicity; and positive regulation of macromolecule metabolic process. Acts upstream of or within positive regulation of cell migration and positive regulation of cell population proliferation. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Biomarker of several diseases, including allergic rhinitis; alopecia universalis; anogenital venereal wart; autoimmune disease (multiple); and liver disease (multiple).

Mouse Il4 Target Information

Mouse Il4 Predicted Functions

Enables cytokine activity. Involved in several processes, including innate immune response in mucosa; negative regulation of white fat cell proliferation; and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation; positive regulation of macromolecule metabolic process; and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain; colon; hemolymphoid system; liver; and placenta. Used to study Sjogren’s syndrome; atopic dermatitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).

Rat Il4 Target Information

Target Symbol: Il4, interleukin 4
Alias:
- B-cell growth factor 1
- B-cell IGG differentiation factor
- B-cell stimulatory factor 1
- BSF-1
- IL-4
- Il4e12
- interleukin-4
- lymphocyte stimulatory factor 1
NCBI_Gene: 287287
- UniprotKB: A0A0G2JVY1
- UniprotKB: P20096

Rat Il4 Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).