EK1112HS

Human PIGF High Sensitivity ELISA Kit

$266.00$380.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.30 pg/mL
Array Range3.13 pg/mL – 200 pg/mL
Assay Time3.5 h
Recovery82% – 104%
Average Recovery93%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.30 pg/mL
Array Range3.13 pg/mL – 200 pg/mL
Assay Time3.5 h
Recovery82% – 104%
Average Recovery93%
Intra Precision1.6% – 3.1%
Inter Precision2.6% – 3.4%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PIGFA monoclonal antibody
Human PIGFA freeze-dried standard
PIGFA detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PIGF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the PIGF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PIGF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

pgf

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