Human PIGF ELISA Kit

$245.00 – $350.00

Related Target	pgf
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.67 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	95% – 122%
Average Recovery	111%

ELISA Kit Detail Information

Related Target	pgf
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	1.67 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	95% – 122%
Average Recovery	111%
Intra Precision	2.9% – 3.8%
Inter Precision	1.9% – 5.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PIGF monoclonal antibody Human PIGF freeze-dried standard PIGF detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PIGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PIGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of PIGF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

pgf

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