Human GM-CSF High Sensitivity ELISA Kit
$266.00 – $380.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 0.07 pg/mL |
| Array Range | 0.16 pg/mL – 10 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 101% |
| Average Recovery | 87% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 0.07 pg/mL |
| Array Range | 0.16 pg/mL – 10 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 101% |
| Average Recovery | 87% |
| Intra Precision | 4.6% – 5.5% |
| Inter Precision | 4.8% – 5.5% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-GM-CSF monoclonal antibody Human GM-CSF freeze-dried standard GM-CSF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human GM-CSF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the GM-CSF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of GM-CSF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
CSF2
CSF2 Target Infomation Overview
- Target Symbol: CSF2, colony stimulating factor 2
- Alias: GM-CSF; GMCSF
- Alias Names: sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)
CSF2, colony stimulating factor 2 Target Infomation by Species
- Human
- Mouse
- Rat
Human CSF2 Target Information
- Target Symbol: CSF2, colony stimulating factor 2
- Alias:
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- GMCSF
- granulocyte macrophage-colony stimulating factor
- granulocyte-macrophage colony stimulating factor
- granulocyte-macrophage colony-stimulating factor
- MGC131935
- MGC138897
- molgramostim
- molgramostin
- sargramostim
- NCBI_Gene: 1437
- UniProtKB: P04141
Human CSF2 Predicted Functions
Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.
Mouse Csf2 Target Information
- Target Symbol: Csf2, colony stimulating factor 2 (granulocyte-macrophage)
- Alias:
- colony stimulating factor, granulocyte macrophage
- Csfgm
- Gm-CSf
- GMCSF
- MGI-IGM
- NCBI_Gene: 12981
Mouse Csf2 Predicted Functions
Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; labyrinthine zone; liver; and lung. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
Rat Csf2 Target Information
- Target Symbol: Csf2, colony stimulating factor 2
- Alias:
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- Gm-csf
- Gmcsf
- granulocyte-macrophage colony-stimulating factor
- NCBI_Gene: 116630
- UniProtKB: P48750
Rat Csf2 Predicted Functions
Enables cytokine activity. Involved in several processes, including cellular response to lipopolysaccharide; macrophage activation; and response to silicon dioxide. Located in extracellular space. Used to study Parkinsonism and myocardial infarction. Biomarker of abdominal aortic aneurysm; interstitial cystitis; and lung disease (multiple). Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
Products with the Same Target - CSF2
Citations for Human GM-CSF High Sensitivity ELISA Kit
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Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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