EasyGo!™ Mouse GM-CSF One-Step ELISA Kit

EK263EG

EasyGo!™ Mouse GM-CSF One-Step ELISA Kit

$349.00 – $499.00

 DATASHEET
Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20uL
Sensitivity0.10pg/ml
Array Range2.34-150pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
SKU: EK263EG Category:

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20uL
Sensitivity0.10pg/ml
Array Range2.34-150pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
Intra Precision< 10%
Inter Precision< 10%
PlateDetachable 96-well Plate
Storage2-8℃
Deliveryblue ice
ComponentsCoated Microplate
standard
Assay Buffer (10×)
TMB
Stop Solution
Washing Buffer (20×)
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

CSF2

CSF2 Target Infomation Overview

  • Target Symbol: CSF2, colony stimulating factor 2
  • Alias: GM-CSF; GMCSF
  • Alias Names: sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)

CSF2, colony stimulating factor 2 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human CSF2 Target Information

  • Target Symbol: CSF2, colony stimulating factor 2
  • Alias:
    • colony stimulating factor 2 (granulocyte-macrophage)
    • colony-stimulating factor
    • CSF
    • GMCSF
    • granulocyte macrophage-colony stimulating factor
    • granulocyte-macrophage colony stimulating factor
    • granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • molgramostim
    • molgramostin
    • sargramostim
  • NCBI_Gene: 1437
  • UniProtKB: P04141

Human CSF2 Predicted Functions

Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.

Mouse Csf2 Target Information

Mouse Csf2 Predicted Functions

Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; labyrinthine zone; liver; and lung. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).

Rat Csf2 Target Information

  • Target Symbol: Csf2, colony stimulating factor 2
  • Alias:
    • colony stimulating factor 2 (granulocyte-macrophage)
    • colony-stimulating factor
    • CSF
    • Gm-csf
    • Gmcsf
    • granulocyte-macrophage colony-stimulating factor
  • NCBI_Gene: 116630
  • UniProtKB: P48750

Rat Csf2 Predicted Functions

Enables cytokine activity. Involved in several processes, including cellular response to lipopolysaccharide; macrophage activation; and response to silicon dioxide. Located in extracellular space. Used to study Parkinsonism and myocardial infarction. Biomarker of abdominal aortic aneurysm; interstitial cystitis; and lung disease (multiple). Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).

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Human GM-CSF High Sensitivity ELISA Kit

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Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

Continue Reading Competitive ELISA: Principles, Methods, and Key Differences

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