Name: Human IL-8 High Sensitivity ELISA Kit
SKU: EK108HS
Availability: InStock

EK108HS

Human IL-8 High Sensitivity ELISA Kit

$266.00 – $380.00

Related Target	CXCL8
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL, cell culture supernatant: 100 μL
Sensitivity	0.28 pg/mL
Array Range	2.34 pg/mL – 150 pg/mL
Assay Time	3.5 h
Recovery	91% – 114%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	CXCL8
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL, cell culture supernatant: 100 μL
Sensitivity	0.28 pg/mL
Array Range	2.34 pg/mL – 150 pg/mL
Assay Time	3.5 h
Recovery	91% – 114%
Average Recovery	102%
Intra Precision	4.3% – 5.0%
Inter Precision	3.3% – 4.7%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-8 monoclonal antibody Human IL-8 high sensitivity freeze-dried standard IL-8 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-8 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-8 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-8 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CXCL8

No description available.

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