EK110HS

Human IL-10 High Sensitivity ELISA Kit

$266.00$380.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.10 pg/mL
Array Range0.39 pg/mL – 25 pg/mL
Assay Time3.5 h
Recovery88% – 100%
Average Recovery92%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.10 pg/mL
Array Range0.39 pg/mL – 25 pg/mL
Assay Time3.5 h
Recovery88% – 100%
Average Recovery92%
Intra Precision3.5% – 4.1%
Inter Precision4.0% – 8.8%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody
Human IL-10 freeze-dried standard
IL-10 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-10 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-10 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL10

IL10 Target Infomation Overview

  • Target Symbol: IL10, interleukin 10
  • Gene Groups: Interleukins
  • Alias: CSIF; TGIF; IL10A; IL-10
  • Alias Names: cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10, interleukin 10 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL10 Target Information

  • Target Symbol: IL10, interleukin 10
  • Alias:
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • NCBI_Gene: 3586
  • UniProtKB: P22301

Human IL10 Predicted Functions

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

Mouse Il10 Target Information

Mouse Il10 Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

Rat Il10 Target Information

Rat Il10 Predicted Functions

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

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