Human IL-10 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | Serum, plasma: 50 μL, cell culture supernatant: 100 μL |
| Sensitivity | 0.43 pg/mL |
| Array Range | 3.13 pg/mL – 200 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 117% |
| Average Recovery | 104% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | Serum, plasma: 50 μL, cell culture supernatant: 100 μL |
| Sensitivity | 0.43 pg/mL |
| Array Range | 3.13 pg/mL – 200 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 117% |
| Average Recovery | 104% |
| Intra Precision | 3.2% – 5.7% |
| Inter Precision | 2.9% – 8.2% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody Human IL-10 freeze-dried standard IL-10 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-10 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-10 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL10
IL10 Target Infomation Overview
- Target Symbol: IL10, interleukin 10
- Gene Groups: Interleukins
- Alias: CSIF; TGIF; IL10A; IL-10
- Alias Names: cytokine synthesis inhibitory factor; T-cell growth inhibitory factor
IL10, interleukin 10 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL10 Target Information
- Target Symbol: IL10, interleukin 10
- Alias:
- CSIF
- cytokine synthesis inhibitory factor
- GVHDS
- IL-10
- IL10A
- interleukin-10
- MGC126450
- MGC126451
- T-cell growth inhibitory factor
- TGIF
- NCBI_Gene: 3586
- UniProtKB: P22301
Human IL10 Predicted Functions
Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).
Mouse Il10 Target Information
- Target Symbol: Il10, interleukin 10
- Alias:
- cytokine synthesis inhibitory factor
- IL-10
- Il-10
- NCBI_Gene: 16153
Mouse Il10 Predicted Functions
Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).
Rat Il10 Target Information
- Target Symbol: Il10, interleukin 10
- Alias:
- CSIF
- cytokine synthesis inhibitory factor
- If2a
- IL-10
- IL10X
- interferon 2a
- interleukin-10
- NCBI_Gene: 25325
Rat Il10 Predicted Functions
Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).
Products with the Same Target - IL10
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