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Human GM-CSF High Sensitivity ELISA Kit
$380.00 – $480.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 0.07 pg/mL |
| Range | 0.16 pg/mL – 10 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 101% |
| Average Recovery | 0.87 |
| Intra Precision | 4.6% – 5.5% |
| Inter-Precision | 4.8% – 5.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-GM-CSF monoclonal antibody Human GM-CSF freeze-dried standard GM-CSF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human GM-CSF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the GM-CSF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of GM-CSF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CSF2
CSF2 Target Infomation Overview
- Target Symbol: CSF2, colony stimulating factor 2
- Alias: GM-CSF; GMCSF
- Alias Names: sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)
CSF2, colony stimulating factor 2 Target Infomation by Species
HumanMouseRat
Human CSF2 Target Information
- Target Symbol: CSF2, colony stimulating factor 2
- Alias:
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- GMCSF
- granulocyte macrophage-colony stimulating factor
- granulocyte-macrophage colony stimulating factor
- granulocyte-macrophage colony-stimulating factor
- MGC131935
- MGC138897
- molgramostim
- molgramostin
- sargramostim
- NCBI_Gene: 1437
- UniProtKB: P04141
Human CSF2 Predicted Functions
Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.
Mouse Csf2 Target Information
- Target Symbol: Csf2, colony stimulating factor 2 (granulocyte-macrophage)
- Alias:
- colony stimulating factor, granulocyte macrophage
- Csfgm
- Gm-CSf
- GMCSF
- MGI-IGM
- NCBI_Gene: 12981
Mouse Csf2 Predicted Functions
Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; labyrinthine zone; liver; and lung. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
Rat Csf2 Target Information
- Target Symbol: Csf2, colony stimulating factor 2
- Alias:
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- Gm-csf
- Gmcsf
- granulocyte-macrophage colony-stimulating factor
- NCBI_Gene: 116630
- UniProtKB: P48750
Rat Csf2 Predicted Functions
Enables cytokine activity. Involved in several processes, including cellular response to lipopolysaccharide; macrophage activation; and response to silicon dioxide. Located in extracellular space. Used to study Parkinsonism and myocardial infarction. Biomarker of abdominal aortic aneurysm; interstitial cystitis; and lung disease (multiple). Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
