Human IL-6 High Sensitivity ELISA Kit

$380.00$480.00

TARGETS IL6
SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.02 pg/mL
Range 0.31 pg/mL – 20 pg/mL
Assay Time 3.5 h
Recovery 74% – 119%
Average Recovery 0.94
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.02 pg/mL
Range 0.31 pg/mL – 20 pg/mL
Assay Time 3.5 h
Recovery 74% – 119%
Average Recovery 0.94
Intra Precision 4.7% – 5.6%
Inter-Precision 4.6% – 6.7%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Human IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL6

IL6 Target Infomation Overview

  • Target Symbol: IL6, interleukin 6
  • Gene Groups: Interferons; Interleukins; Interleukin 6 type cytokine family
  • Alias: IL-6; BSF2; HGF; HSF
  • Previous Names: IFNB2
  • Alias Names: interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6, interleukin 6 Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human IL6 Target Information

  • Target Symbol: IL6, interleukin 6
  • Alias:
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • NCBI_Gene: 3569
  • UniProtKB: P05231

Human IL6 Predicted Functions

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Il6 Target Information

Mouse Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Il6 Target Information

  • Target Symbol: Il6, interleukin 6
  • Alias:
    • Ifnb2
    • IL-6
    • ILg6
    • Interleukin 6 (interferon beta 2)
    • Interleukin 6 (interferon, beta 2)
    • interleukin-6
  • NCBI_Gene: 24498
  • UniProtKB: P20607

Rat Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

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IL6 Target News

[catlist tags=”il6″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

7.7
Extraction, separation and efficacy of yam polysaccharide
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
6.6
Metabolic Reprogramming of CD4+ T Cells by Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuates Autoimmune Hepatitis Through Mitochondrial Protein Transfer
International Journal of Nanomedicine
1.8
Potential Action Mechanism of Erianin in Relieving MNNG-triggered Chronic Atrophic Gastritis
CELL BIOCHEMISTRY AND BIOPHYSICS
4.9
Silencing ARL11 relieved atherosclerotic inflammation and lipid deposition via retraining JAK2/STAT1 pathway
ATHEROSCLEROSIS
7.9
Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17 cells
JOURNAL OF AUTOIMMUNITY
6
Negative impact of maternal depressive symptoms on infancy neurodevelopment: a moderated mediation effect of maternal inflammation
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY
2.6
Invasion of human dental pulp fibroblasts by Porphyromonas gingivalis leads to autophagy via the phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling pathway
Journal of Oral Biosciences
13.3
Spatio-temporally controlled all-in-one hydrogel microspheres for ulcerative colitis treatment based on cocktail strategy
CHEMICAL ENGINEERING JOURNAL
7.1
A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation
FREE RADICAL BIOLOGY AND MEDICINE
1.4
Effects of continuous negative pressure suction combined with autologous platelet-rich gel on the levels of CRP, IL-6, wound healing and length of stay in clients with diabetic foot
TRANSFUSION AND APHERESIS SCIENCE
18.8
A citrullinated antigenic vaccine in treatment of autoimmune arthritis
Science Bulletin
2.1
Correlation between matrix metalloproteinase-2, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinases-1 and white matter hyperintensities in patients with cerebral small vessel disease based on cranial magnetic resonance 3D imaging
MAGNETIC RESONANCE IMAGING
3.1
STING-mediated IL-6 Inhibits OATP1B1 Expression via the TCF4 Signaling Pathway in Cholestasis
Journal of Clinical and Translational Hepatology
4.8
The protective role of Cordyceps cicadae and its active ingredient myriocin against sodium iodate-induced age-related macular degeneration via an anti-necroptotic TNF-RIPK1/3 pathway
JOURNAL OF ETHNOPHARMACOLOGY
2
Network pharmacology and experimental validation to explore the anti-aging mechanism of action of betulinic acid
Network Modeling and Analysis in Health Informatics and Bioinformatics
3.4
The effects of metabolites from three vaginal bacteria on the Syndecan-1 of cervical epithelial cells
Heliyon
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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