Name: Human IL-33 High Sensitivity ELISA Kit
SKU: EK133HS
Availability: InStock

EK133HS

Human IL-33 High Sensitivity ELISA Kit

$319.00 – $449.00

Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	0.16 pg/mL
Array Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	92% – 100%
Average Recovery	99%

SKU: EK133HS Category: High Sensitivity ELISA Kit

ELISA Kit Detail Information

Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	0.16 pg/mL
Array Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	92% – 100%
Average Recovery	99%
Intra Precision	4.0% – 4.6%
Inter Precision	2.6% – 4.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-33 monoclonal antibody Human IL-33 high sensitivity freeze-dried standard IL-33 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-33 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-33 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-33 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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