Name: Mouse IL-12p70 High Sensitivity ELISA Kit
SKU: EK212HS
Availability: InStock

EK212HS

Mouse IL-12p70 High Sensitivity ELISA Kit

$319.00 – $449.00

Related Target	il12
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.57 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	92% – 115%
Average Recovery	104%

SKU: EK212HS Category: High Sensitivity ELISA Kit

ELISA Kit Detail Information

Related Target	il12
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.57 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	92% – 115%
Average Recovery	104%
Intra Precision	2.2% – 3.1%
Inter Precision	2.6% – 3.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-12p70 monoclonal antibody Mouse IL-12p70 freeze-dried standard IL-12p70 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-12p70 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-12p70 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-12p70 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

il12

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