EK106HS

Human IL-6 High Sensitivity ELISA Kit

$266.00$380.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.02 pg/mL
Array Range0.31 pg/mL – 20 pg/mL
Assay Time3.5 h
Recovery74% – 119%
Average Recovery94%
-+
SKU: EK106HS Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.02 pg/mL
Array Range0.31 pg/mL – 20 pg/mL
Assay Time3.5 h
Recovery74% – 119%
Average Recovery94%
Intra Precision4.7% – 5.6%
Inter Precision4.6% – 6.7%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Human IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL6

IL6 Target Infomation Overview

  • Target Symbol: IL6, interleukin 6
  • Gene Groups: Interferons; Interleukins; Interleukin 6 type cytokine family
  • Alias: IL-6; BSF2; HGF; HSF
  • Previous Names: IFNB2
  • Alias Names: interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6, interleukin 6 Target Infomation by Species

  • human iconHuman
  • mouse iconMouse
  • rat iconRat

Human IL6 Target Information

  • Target Symbol: IL6, interleukin 6
  • Alias:
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • NCBI_Gene: 3569
  • UniProtKB: P05231

Human IL6 Predicted Functions

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

Mouse Il6 Target Information

Mouse Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

Rat Il6 Target Information

  • Target Symbol: Il6, interleukin 6
  • Alias:
    • Ifnb2
    • IL-6
    • ILg6
    • Interleukin 6 (interferon beta 2)
    • Interleukin 6 (interferon, beta 2)
    • interleukin-6
  • NCBI_Gene: 24498
  • UniProtKB: P20607

Rat Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).