Human IL-9 High Sensitivity ELISA Kit

High Sensitivity ELISA

$380.00 – $480.00

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Datasheet

SDS File

TARGETS	IL9
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL；cell culture supernatant: 100 μL
Sensitivity	0.16 pg/mL
Range	0.63 pg/mL – 40 pg/mL
Assay Time	3.5 h
Recovery	98% – 116%
Average Recovery	1.02

Size

48T

96T

Clear

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL；cell culture supernatant: 100 μL
Sensitivity	0.16 pg/mL
Range	0.63 pg/mL – 40 pg/mL
Assay Time	3.5 h
Recovery	98% – 116%
Average Recovery	1.02
Intra Precision	3.8% – 4.3%
Inter-Precision	3.9% – 4.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-9 monoclonal antibody Human IL-9 high sensitivity freeze-dried standard IL-9 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-9 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-9 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL9

IL9 Target Infomation Overview

Target Symbol: IL9, interleukin 9
Gene Groups: Interleukins
Alias: IL-9; HP40; P40
Alias Names: p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40

IL9, interleukin 9 Target Infomation by Species

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Human IL9 Target Information

Target Symbol: IL9, interleukin 9
Alias:
- cytokine P40
- homolog of mouse T cell and mast cell growth factor 40
- HP40
- IL-9
- interleukin-9
- P40
- p40 cytokine
- p40 T-cell and mast cell growth factor
- T-cell growth factor p40
NCBI_Gene: 3578
UniProtKB: P15248

Human IL9 Predicted Functions

Predicted to enable cytokine activity and interleukin-9 receptor binding activity. Predicted to be involved in positive regulation of interleukin-5 production. Predicted to act upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in asthma and respiratory syncytial virus infectious disease. Biomarker of COVID-19; asthma; cystic fibrosis; lung disease (multiple); and rhinitis.

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Mouse Il9 Target Information

Target Symbol: Il9, interleukin 9
Alias:
- Il-9
- P40
NCBI_Gene: 16198
- UniprotKB: P15247

Mouse Il9 Predicted Functions

Enables cytokine activity. Involved in positive regulation of interleukin-5 production. Acts upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Is expressed in gut; liver; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma and respiratory syncytial virus infectious disease. Orthologous to human IL9 (interleukin 9).

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Rat Il9 Target Information

Target Symbol: Il9, interleukin 9
Alias:
- interleukin-9
NCBI_Gene: 116558
UniProtKB: D4A8I9

Rat Il9 Predicted Functions

Enables cytokine activity and interleukin-9 receptor binding activity. Involved in positive regulation of cell growth. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in asthma and respiratory syncytial virus infectious disease. Orthologous to human IL9 (interleukin 9).

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