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Human IL-9 High Sensitivity ELISA Kit
$380.00 – $480.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 20 μL；cell culture supernatant: 100 μL |
| Sensitivity | 0.16 pg/mL |
| Range | 0.63 pg/mL – 40 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 98% – 116% |
| Average Recovery | 1.02 |
| Intra Precision | 3.8% – 4.3% |
| Inter-Precision | 3.9% – 4.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-9 monoclonal antibody Human IL-9 high sensitivity freeze-dried standard IL-9 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-9 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-9 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL9
IL9 Target Infomation Overview
- Target Symbol: IL9, interleukin 9
- Gene Groups: Interleukins
- Alias: IL-9; HP40; P40
- Alias Names: p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40
IL9, interleukin 9 Target Infomation by Species
HumanMouseRat
Human IL9 Target Information
- Target Symbol: IL9, interleukin 9
- Alias:
- cytokine P40
- homolog of mouse T cell and mast cell growth factor 40
- HP40
- IL-9
- interleukin-9
- P40
- p40 cytokine
- p40 T-cell and mast cell growth factor
- T-cell growth factor p40
- NCBI_Gene: 3578
- UniProtKB: P15248
Human IL9 Predicted Functions
Predicted to enable cytokine activity and interleukin-9 receptor binding activity. Predicted to be involved in positive regulation of interleukin-5 production. Predicted to act upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in asthma and respiratory syncytial virus infectious disease. Biomarker of COVID-19; asthma; cystic fibrosis; lung disease (multiple); and rhinitis.
Mouse Il9 Target Information
- Target Symbol: Il9, interleukin 9
- Alias:
- Il-9
- P40
- NCBI_Gene: 16198
Mouse Il9 Predicted Functions
Enables cytokine activity. Involved in positive regulation of interleukin-5 production. Acts upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Is expressed in gut; liver; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma and respiratory syncytial virus infectious disease. Orthologous to human IL9 (interleukin 9).
Rat Il9 Target Information
- Target Symbol: Il9, interleukin 9
- Alias:
- interleukin-9
- NCBI_Gene: 116558
- UniProtKB: D4A8I9
Rat Il9 Predicted Functions
Enables cytokine activity and interleukin-9 receptor binding activity. Involved in positive regulation of cell growth. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in asthma and respiratory syncytial virus infectious disease. Orthologous to human IL9 (interleukin 9).
