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Human PD-L1/B7-H1/CD274 High Sensitivity ELISA Kit
$380.00 – $480.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 0.49 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92% – 115% |
| Average Recovery | 1.04 |
| Intra Precision | 3.8% – 4.4% |
| Inter-Precision | 4.1% – 4.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PD-L1 monoclonal antibody Human PD-L1 freeze-dried standard PD-L1 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PD-L1单克隆 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the PD-L1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PD-L1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CD274
CD274 Target Infomation Overview
- Target Symbol: CD274, CD274 molecule
- Gene Groups: CD molecules; Receptor ligands; V-set domain containing; C2-set domain containing; B7 family
- Alias: B7-H; B7H1; PD-L1; PDL1; B7-H1
- Previous Names: PDCD1LG1
- Alias Names: B7 homolog 1; programmed cell death 1 ligand 1; CD274 antigen
CD274, CD274 molecule Target Infomation by Species
HumanMouseRat
Human CD274 Target Information
- Target Symbol: CD274, CD274 molecule
- Alias:
- B7 homolog 1
- B7-H
- B7H1
- CD274 antigen
- hPD-L1
- MGC142294
- MGC142296
- PD-L1
- PDCD1 ligand 1
- PDCD1L1
- PDCD1LG1
- PDL1
- programmed cell death 1 ligand 1
- programmed death ligand 1
- NCBI_Gene: 29126
- UniProtKB: Q9NZQ7
Human CD274 Predicted Functions
Involved in several processes, including positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process; regulation of T cell activation; and regulation of cytokine production. Located in several cellular components, including endosome membrane; extracellular exosome; and nucleoplasm. Biomarker of several diseases, including Cryptococcal meningitis; Human papillomavirus infectious disease; gastrointestinal system cancer (multiple); lymphoma (multiple); and tuberculosis (multiple).
Mouse Cd274 Target Information
- Target Symbol: Cd274, CD274 antigen
- Alias:
- B7-H1
- PD-L1
- Pdcd1lg1
- programmed cell death 1 ligand 1
- NCBI_Gene: 60533
Mouse Cd274 Predicted Functions
Acts upstream of or within negative regulation of T cell proliferation and toxin transport. Located in external side of plasma membrane. Is expressed in retina and thymus primordium. Orthologous to human CD274 (CD274 molecule).
Rat Cd274 Target Information
- Target Symbol: Cd274, CD274 molecule
- Alias:
- CD274 antigen
- LOC499342
- Pdcd1lg1
- programmed cell death 1 ligand 1
- RGD1566211
- similar to PD-1-ligand precursor
- NCBI_Gene: 499342
- UniProtKB: D4AE25
Rat Cd274 Predicted Functions
Involved in positive regulation of T cell proliferation and positive regulation of cell migration. Located in cell surface. Biomarker of periodontitis and pre-eclampsia. Orthologous to human CD274 (CD274 molecule).
