Human PD-L1/B7-H1/CD274 High Sensitivity ELISA Kit

$380.00$480.00

Download:
TARGETSCD274
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.49 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery92% – 115%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.49 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery92% – 115%
Average Recovery1.04
Intra Precision3.8% – 4.4%
Inter-Precision4.1% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PD-L1 monoclonal antibody
Human PD-L1 freeze-dried standard
PD-L1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PD-L1单克隆 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the PD-L1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PD-L1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CD274

CD274 Target Infomation Overview

  • Target Symbol: CD274, CD274 molecule
  • Gene Groups: CD molecules; Receptor ligands; V-set domain containing; C2-set domain containing; B7 family
  • Alias: B7-H; B7H1; PD-L1; PDL1; B7-H1
  • Previous Names: PDCD1LG1
  • Alias Names: B7 homolog 1; programmed cell death 1 ligand 1; CD274 antigen

CD274, CD274 molecule Target Infomation by Species

HumanMouseRat

Human CD274 Target Information

  • Target Symbol: CD274, CD274 molecule
  • Alias:
    • B7 homolog 1
    • B7-H
    • B7H1
    • CD274 antigen
    • hPD-L1
    • MGC142294
    • MGC142296
    • PD-L1
    • PDCD1 ligand 1
    • PDCD1L1
    • PDCD1LG1
    • PDL1
    • programmed cell death 1 ligand 1
    • programmed death ligand 1
  • NCBI_Gene: 29126
  • UniProtKB: Q9NZQ7

Human CD274 Predicted Functions

Involved in several processes, including positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process; regulation of T cell activation; and regulation of cytokine production. Located in several cellular components, including endosome membrane; extracellular exosome; and nucleoplasm. Biomarker of several diseases, including Cryptococcal meningitis; Human papillomavirus infectious disease; gastrointestinal system cancer (multiple); lymphoma (multiple); and tuberculosis (multiple).

Mouse Cd274 Target Information

Mouse Cd274 Predicted Functions

Acts upstream of or within negative regulation of T cell proliferation and toxin transport. Located in external side of plasma membrane. Is expressed in retina and thymus primordium. Orthologous to human CD274 (CD274 molecule).

Rat Cd274 Target Information

  • Target Symbol: Cd274, CD274 molecule
  • Alias:
    • CD274 antigen
    • LOC499342
    • Pdcd1lg1
    • programmed cell death 1 ligand 1
    • RGD1566211
    • similar to PD-1-ligand precursor
  • NCBI_Gene: 499342
  • UniProtKB: D4AE25

Rat Cd274 Predicted Functions

Involved in positive regulation of T cell proliferation and positive regulation of cell migration. Located in cell surface. Biomarker of periodontitis and pre-eclampsia. Orthologous to human CD274 (CD274 molecule).

CD274 Target News

    Multiple Sclerosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

    Related Products

    more +

    $380.00$480.00

    $380.00$480.00

    $380.00$480.00

    Same Target

    EK2261

    $350.00$450.00

    EK1261

    $350.00$450.00