Name: Mouse IL-33 High Sensitivity ELISA Kit
SKU: EK233HS
Availability: InStock

EK233HS

Mouse IL-33 High Sensitivity ELISA Kit

$266.00 – $380.00

Related Target	IL33
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	0.26 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	80% – 102%
Average Recovery	87%

SKU: EK233HS Category: High Sensitivity ELISA Kit

ELISA Kit Detail Information

Related Target	IL33
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	0.26 pg/mL
Array Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	80% – 102%
Average Recovery	87%
Intra Precision	2.2% – 3.2%
Inter Precision	2.6% – 3.5%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-33 monoclonal antibody Mouse IL-33 high sensitivity freeze-dried standard IL-33 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-33 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-33 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-33 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL33

IL33 Target Infomation Overview

Target Symbol: IL33, interleukin 33
Gene Groups: Interleukins
Alias: DVS27; DKFZp586H0523; NF-HEV; IL1F11
Previous Names: C9orf26
Alias Names: DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)

IL33, interleukin 33 Target Infomation by Species

Human
Mouse
Rat

Human IL33 Target Information

Target Symbol: IL33, interleukin 33
Alias:
- C9orf26
- DKFZp586H0523
- DVS27
- DVS27-related protein
- IL-1F11
- IL-33
- IL1F11
- interleukin-1 family member 11
- interleukin-33
- NF-HEV
- NFEHEV
- nuclear factor for high endothelial venules
- nuclear factor from high endothelial venules
- RP11-575C20.2
NCBI_Gene: 90865
UniProtKB: O95760

Human IL33 Predicted Functions

Enables cytokine activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of chemokine production; and positive regulation of macrophage activation. Located in nucleoplasm. Part of cytoplasm and nucleus. Implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Biomarker of several diseases, including colitis (multiple); common cold; gout; liver disease (multiple); and tuberculosis (multiple).

Mouse Il33 Target Information

Target Symbol: Il33, interleukin 33
Alias:
- 9230117N10Rik
- Il-33
- Il1f11
- NF-HEV
- RIKEN cDNA 9230117N10 gene
NCBI_Gene: 77125

Mouse Il33 Predicted Functions

Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer’s disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

Rat Il33 Target Information

Target Symbol: Il33, interleukin 33
Alias:
- IL-33
- interleukin-33
- LOC361749
- RGD1311155
- similar to RIKEN cDNA 9230117N10
NCBI_Gene: 361749
- UniprotKB: A0A096MIT4
- UniprotKB: Q66H70

Rat Il33 Predicted Functions

Predicted to enable cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including cellular response to mechanical stimulus; positive regulation of oligodendrocyte differentiation; and regulation of sensory perception of pain. Located in nucleus. Biomarker of acute myocardial infarction; colon cancer; and diabetes mellitus. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

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