Mouse IL-33 High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSIL33
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.26 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery80% – 102%
Average Recovery0.87
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.26 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery80% – 102%
Average Recovery0.87
Intra Precision2.2% – 3.2%
Inter-Precision2.6% – 3.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-33 monoclonal antibody
Mouse IL-33 high sensitivity freeze-dried standard
IL-33 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-33 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-33 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-33 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL33

IL33 Target Infomation Overview

  • Target Symbol: IL33, interleukin 33
  • Gene Groups: Interleukins
  • Alias: DVS27; DKFZp586H0523; NF-HEV; IL1F11
  • Previous Names: C9orf26
  • Alias Names: DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)

IL33, interleukin 33 Target Infomation by Species

HumanMouseRat

Human IL33 Target Information

  • Target Symbol: IL33, interleukin 33
  • Alias:
    • C9orf26
    • DKFZp586H0523
    • DVS27
    • DVS27-related protein
    • IL-1F11
    • IL-33
    • IL1F11
    • interleukin-1 family member 11
    • interleukin-33
    • NF-HEV
    • NFEHEV
    • nuclear factor for high endothelial venules
    • nuclear factor from high endothelial venules
    • RP11-575C20.2
  • NCBI_Gene: 90865
  • UniProtKB: O95760

Human IL33 Predicted Functions

Enables cytokine activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of chemokine production; and positive regulation of macrophage activation. Located in nucleoplasm. Part of cytoplasm and nucleus. Implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Biomarker of several diseases, including colitis (multiple); common cold; gout; liver disease (multiple); and tuberculosis (multiple).

Mouse Il33 Target Information

Mouse Il33 Predicted Functions

Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer’s disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

Rat Il33 Target Information

Rat Il33 Predicted Functions

Predicted to enable cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including cellular response to mechanical stimulus; positive regulation of oligodendrocyte differentiation; and regulation of sensory perception of pain. Located in nucleus. Biomarker of acute myocardial infarction; colon cancer; and diabetes mellitus. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

IL33 Target News

    2.4
    The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
    PARASITOLOGY
    5.4
    Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
    JOURNAL OF ETHNOPHARMACOLOGY
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Osteoarthrosis & Rheumatoid Arthritis
    Systemic Lupus Erythematosus

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