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Mouse IL-33 High Sensitivity ELISA Kit
$380.00 – $480.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | Serum, plasma: 20 μL；cell culture supernatant: 100 μL |
| Sensitivity | 0.26 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 102% |
| Average Recovery | 0.87 |
| Intra Precision | 2.2% – 3.2% |
| Inter-Precision | 2.6% – 3.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-33 monoclonal antibody Mouse IL-33 high sensitivity freeze-dried standard IL-33 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-33 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-33 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-33 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL33
IL33 Target Infomation Overview
- Target Symbol: IL33, interleukin 33
- Gene Groups: Interleukins
- Alias: DVS27; DKFZp586H0523; NF-HEV; IL1F11
- Previous Names: C9orf26
- Alias Names: DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)
IL33, interleukin 33 Target Infomation by Species
HumanMouseRat
Human IL33 Target Information
- Target Symbol: IL33, interleukin 33
- Alias:
- C9orf26
- DKFZp586H0523
- DVS27
- DVS27-related protein
- IL-1F11
- IL-33
- IL1F11
- interleukin-1 family member 11
- interleukin-33
- NF-HEV
- NFEHEV
- nuclear factor for high endothelial venules
- nuclear factor from high endothelial venules
- RP11-575C20.2
- NCBI_Gene: 90865
- UniProtKB: O95760
Human IL33 Predicted Functions
Enables cytokine activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of chemokine production; and positive regulation of macrophage activation. Located in nucleoplasm. Part of cytoplasm and nucleus. Implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Biomarker of several diseases, including colitis (multiple); common cold; gout; liver disease (multiple); and tuberculosis (multiple).
Mouse Il33 Target Information
- Target Symbol: Il33, interleukin 33
- Alias:
- 9230117N10Rik
- Il-33
- Il1f11
- NF-HEV
- RIKEN cDNA 9230117N10 gene
- NCBI_Gene: 77125
Mouse Il33 Predicted Functions
Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer’s disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).
Rat Il33 Target Information
- Target Symbol: Il33, interleukin 33
- Alias:
- IL-33
- interleukin-33
- LOC361749
- RGD1311155
- similar to RIKEN cDNA 9230117N10
- NCBI_Gene: 361749
Rat Il33 Predicted Functions
Predicted to enable cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including cellular response to mechanical stimulus; positive regulation of oligodendrocyte differentiation; and regulation of sensory perception of pain. Located in nucleus. Biomarker of acute myocardial infarction; colon cancer; and diabetes mellitus. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).
